Single fibroblast stiffness was assessed from nanoindentation measurements with AFM, as reported in [15 (link)]. In brief, a stand-alone AFM (Bioscope, Veeco, Plainview, NY, USA) was adapted to an optical microscope and provided with a low-spring constant (0.03 nN/nm) AFM cantilever (Veeco) was used to assess Young’s modulus (E) of single fibroblasts. For this purpose, three force versus displacement curves (F vs. z) were recorded on the perinuclear region of each fibroblast at a moderate loading force (<1 nN) and low speed (~5 µm/s). E of single fibroblasts was computed by fitting a suitable contact elastic model to each F vs. z curve and averaging it over three recordings. Final E for a fibroblast population for each donor and experimental condition was calculated from at least nine measurements as described [32 (link)].
Afm cantilever
Manufactured by Veeco
Sourced in United States
The AFM cantilever is a critical component in an atomic force microscope (AFM) system. Its primary function is to act as a probe that interacts with the sample surface, enabling the measurement of surface topography and other physical properties at the nanoscale level.
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Lab products found in correlation
2 protocols using afm cantilever
1
Nanoindentation for Fibroblast Stiffness
2
Measuring Cellular Elasticity via AFM
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Cells were cultured on glass-bottomed 35-mm Petri dishes (MatTek) and placed on the stage of an inverted optical microscope (Olympus IX70-S8F2) equipped with a microinbucator (HCMIS ALA Science) to hold the temperature at 37°C. A commercial stand-alone AFM (Bioscope, Veeco) adapted to the optical microscope and provided with a low-spring constant (0.03 nN/nm) AFM cantilever (Veeco) was used to assess the Young’s modulus (E) of single cells, following a protocol described in detail elsewhere (Alcaraz et al., 2003 (link), 2011 (link)). The spring constant of the cantilever was calibrated using the thermal fluctuations method (Roca-Cusachs et al., 2008 (link)). In brief, three standard force versus displacement curves (F vs. z) were recorded on the perinuclear region of each cell at moderate loading force (∼1 nN) and low speed (∼5 µm/s). The E of a single cell was computed by fitting a suitable contact elastic model to each F-versus-z curve and averaging it over the three recordings. The final E for a cell population in each experimental condition was calculated from at least nine measurements for each independent experiment (n ≥ 2).
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