Anti mouse and anti rabbit igg peroxidase or fitc conjugated antibodies

The polyclonal antibodies specific for ERα (H-184), ERβ (H-150), SIRT1, p300 and the monoclonal antibodies specific for PARP1, acetylated-lysine, and α-tubulin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-mouse and anti-rabbit IgG peroxidase or FITC-conjugated antibodies, the proteasome inhibitor MG132, the selective SIRT1 inhibitor EX527 and chemical reagents were from Sigma-Aldrich (St Louis, MO, USA). ECL, nitrocellulose membranes, and protein assay kit were from Bio-Rad (Hercules, CA, USA). TRIzol^®, culture media, sera, and LipofectAMINE transfection reagent were from Thermo Fisher (Waltham, MA, USA). The ERα-selective agonist PPT and 17β-estradiol were from Tocris Bioscience (Bristol, UK). The ERβ-selective agonist KB9520 was originally provided by Karo Bio AB (Huddinge, SE)^{24 (link)}. Today this ligand is owned by Oasmia Pharmaceuticals AB (Uppsala, SE).

The polyclonal antibodies specific for histone H3 trimethyl lysine 27 (H3K27me3), histone H3, SIRT1, KDM6B and the monoclonal antibodies specific for Poly (ADP-ribose) polymerase 1 (PARP1), acetyl-lysine, HIF2α, BAP1 and α-tubulin were purchased from Santa Cruz Biotechnology (Santa Cruz CA, USA). Polyclonal antibody anti-p16^ink4a was from Cell Signaling Technology (Leiden, The Netherlands). Polyclonal antibody specific for EZH2 was from Active Motif (La Hulpe, Belgium). Anti-mouse and anti-rabbit IgG peroxidase or FITC conjugated antibodies and chemical reagents were from Sigma-Aldrich (St. Louis, MO, USA). ECL, nitrocellulose membranes and protein assay kit were from Bio-Rad (Hercules, CA, USA). Culture media, sera, antibiotics, LipofectAMINE transfection reagent and the monoclonal antibody specific for TG2 (TG100) were from Thermo Fisher (Waltham, MA, USA). EX-527 and the EZH2-selective inhibitor, EPZ-6438, were from Selleckchem (Houston, TX, USA). The highly selective TG2 inhibitor, 1-155 (cell-permeable) was designed and synthesized as previously documented (37 (link), 38 (link)).