Methanesulfonic acid msa

Manufactured by Merck Group

Sourced in Germany

Methanesulfonic acid (MSA) is a colorless, odorless, and hygroscopic liquid chemical commonly used as a laboratory reagent. It is a strong organic acid with a pKa value of around -1.9. MSA is widely utilized in various chemical applications, including as a catalyst, pH adjuster, and synthetic intermediate.

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Methanesulfonic acid (56 citations)

3 protocols using methanesulfonic acid msa

Ion Chromatography Analysis of Medium

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First, 5 mL of medium was collected from each sample, and the concentration of anions (Cl⁻, NO₃⁻, and SO₄²⁻) and cations (Ca²⁺, K⁺, Mg²⁺, and Na⁺) were analyzed on the DX600A ion chromatograph (Dionex, Sunnyvale, CA, USA). The medium were filtered through 25TF PURADISC™ membrane filters (Whatman, Little Chalfont, UK) with a diameter of 0.45 µm. Using the AS40 autosampler, solutions were introduced into IonPac analytical columns. The AS9-HC column (4 × 250 mm) and a liquid phase consisting of a 9 mM solution of Na₂CO₃ were used for the determination of anions. The concentrations of the cations were determined using a CS-12A column (4 × 250 mm) with 20 mM methanesulfonic acid (MSA, Sigma–Aldrich, Munich, Germany) as the eluent. The flow rate of the eluent through the column in both cases was 1 mL/min. The separated ions were identified by the ED50 detector, and the ion concentrations were analyzed using the PeakNet software (Dionex, USA).

Kucała M., Saładyga M, & Kaminski A. (2021). Phytoremediation of CYN, MC-LR and ANTX-a from Water by the Submerged Macrophyte Lemna trisulca. Cells, 10(3), 699.

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Synthesis of PEG-based Polymers

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ε-Caprolactone (ε-CL, 99%, Sigma-Aldrich) was stirred over CaH₂ for 24 h at room temperature and then it was distilled at reduced pressure under nitrogen. α-Methoxy-poly(ethylene glycol)₄₄-ω-hydroxide (MeO–PEG₄₄–OH, Mn = 2000 g/mol) was dried by co-evaporation with anhydrous toluene using a rotary evaporator. Methane sulfonic acid (MSA) was purchased from Sigma-Aldrich, and was used without further purification. Rabbit α-tubulin antibody and mouse β-Actin were purchased from Sigma-Aldrich; rabbit anti-CD31 (GB11063-2) was purchased from Servicebio; rabbit anti-Bcl-2 antibody and rabbit anti-Ki67 were purchased from Bioss; rabbit anti-MDR1/ABCB1 (E1Y7B) antibody was purchased from Cell Signaling Technology; horseradish peroxidase (HRP)-conjugated secondary antibodies were obtained from Cell signaling Technology. LysoTracker^® Red DND-99 and DAPI were obtained from Thermo Scientific. Reactive oxygen species (ROS) detection kit and Annexin V-FITC apoptosis detection kit were purchased from Biovision. ECL western blotting detection kit was purchased from Millipore.

Xu H., Cui W., Zong Z., Tan Y., Xu C., Cao J., Lai T., Tang Q., Wang Z., Sui X, & Wang C. (2022). A facile method for anti-cancer drug encapsulation into polymersomes with a core-satellite structure. Drug Delivery, 29(1), 2414-2427.

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Purification and Preparation of Analytical Standards

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Nitric acid (analytical grade, 65%; Merck KGaA, Darmstadt, Germany) was purified at sub-boiling (s.b.) grade by a PTFE distillation system (DST-1000 Acid Purification System, Savillex, Eden Prairie, MN, US). Water used for standards and sample preparation was of ultrapure grade (type 1) with 18.2 MΩ cm resistivity, produced by a Milli-Q system (Merk-Millipore, Darmstadt, Germany). Intermediate metal standard solutions were prepared from 1000 mg L -1 stock solutions (Sigma-Aldrich TraceCERT, Buchs, Switzerland). For the preparation of the cationic/anionic standards, the following chemicals at purity > 99% have been used: NH 4 Cl (Merck), NaAc (Carlo Erba, Cornaredo, Italy), NaFor (Merck), malonic acid (Aldrich), Na 2 Ox (Carlo Erba), NaNO 3 (Sigma-Aldrich), Na 2 SO 4 (Alfa Aesar, Kandel, Germany), Na 3 PO 4 •12H 2 O (Carlo Erba), and NaNO 2 (Merck). For ion chromatography, KOH (Merck) and methanesulfonic acid (MSA, Sigma-Aldrich) were used for the preparation of the mobile phases. Fe(NO 3 ) 3 •9H 2 O (Carlo Erba, for analysis grade) and K 2 Ox•H 2 O (Carlo Erba, ≥ 99.5%) were used for the voltammetric study of Fe 3+ -Ox system, using NaCl (Merck, for analysis grade) as ionic strength buffer and NaOH (Merck, ≥ 98%) to correct the pH of solutions.

Marafante M., Bertinetti S., Carena L., Fabbri D., Malandrino M., Vione D, & Berto S. (2024). Chemical characterization and speciation of the soluble fraction of Arctic PM₁₀. Analytical and bioanalytical chemistry, 416(6).

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