Tetrachloroauric (III) trihydrate (HAuCl4·3H2O: 99.9%), sodium citrate tribasic dehydrate (≥99%) and hydroquinone (≥99%) were purchased from Sigma-Aldrich. Heterobifunctional polyethylene glycol OPSS-PEG-SVA (3.4 kDa, OPSS-PEG-SVA-3400) were purchased from Laysan Bio Inc. Poly(ethylene glycol) 2-mercaptoethyl ether acetic acid (Thiol-PEG-Carboxyl, 600 Da, PG2-CATH-600) were purchased from Nanocs Inc. Fluorescein isothiocyanate–dextran (FITC-dextran) and WST-1 cell proliferation reagent (05015944001) was purchased from Sigma-Aldrich. Propidium iodide (PI) is purchased from Cayman chemical (14289). Mouse IgG isotype control (Ctrl Ab, 31901) was purchased from Invitrogen. Anti-PAR2 antibody (MAB3949) was purchased from R&D systems. Anti-JAM-A antibody BV16 was kindly provided by Dr. Elisabetta Dejana’s lab.32 (link) Human cerebral microvessel endothelial cells (hCMEC/D3) were purchased from EMD Millipore. HEK293 cell with Ca2+ indicator TN-XXL was kindly provided by Dr. Paul Slesinger’s lab.44 (link) PAR2 agonist 2-aminothiazol-4-yl-LIGRL-NH2 (2AT) was provided by Dr. Theodore Price’s lab.40 (link) Somatostatin (SST) was purchased from Sigma-Aldrich (S9129). Pierce modified lowery protein assay kit was obtained from ThermoFisher Scientific (23240).
Pierce modified lowery protein assay kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Pierce Modified Lowry Protein Assay Kit is a colorimetric assay used for the determination of total protein concentration in a sample. The kit utilizes a copper-based reagent to react with the protein, resulting in a color change that can be measured spectrophotometrically. The assay is compatible with a wide range of protein concentrations and can be performed with a microplate reader or spectrophotometer.
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Lab products found in correlation
Propidium iodide, by Cayman Chemical (1 mentions)
Hydroquinone, by Merck Group (1 mentions)
Somatostatin sst, by Merck Group (1 mentions)
Cell proliferation reagent wst 1, by Merck Group (1 mentions)
Hcmec d3, by Merck Group (1 mentions)
Sodium citrate tribasic dehydrate, by Merck Group (1 mentions)
Tetrachloroauric 3 trihydrate, by Merck Group (1 mentions)
Fluorescein isothiocyanate dextran fitc dextran, by Merck Group (1 mentions)
Opss peg sva, by Laysan Bio (1 mentions)
Penta his antibody, by Qiagen (1 mentions)
2 protocols using pierce modified lowery protein assay kit
1
Functionalized Gold Nanoparticle Synthesis
2
Recombinant Protein Quantification and Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The solubilized protein was quantified using Pierce Modified Lowery Protein Assay Kit (Thermo Scientific, USA). The reactivity of protein established by Western blot using Penta His Antibodies (Qiagen, Germany), as well as with PRRSV-specific antibodies in two separate tests. The binding of recombinant protein and the antibodies were visualized using anti-pig horseradish peroxidase (HRP, Thermos Scientific, USA) conjugate and 3,3′-diaminobenzidine substrate. The final protein solution after quantification and characterization was stored at −80°C for further analysis.
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