Ab155058

Manufactured by Abcam

Sourced in United Kingdom

Ab155058 is a reagent for use in laboratory experiments. It is a 25 ml solution of a specific antibody. The core function of this product is to detect and bind to a specific target molecule or antigen.

Automatically generated - may contain errors

Lab products found in correlation

Ab9482, by Abcam (1 mentions)

2 protocols using ab155058

Western Blot Analysis of BMPR1B and GAPDH

Cited 1 time

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Antibodies for BMPR1B (ab155058) and GAPDH (ab9482) were obtained from Abcam (UK). Western blotting was performed according to a previously reported method (Liu et al., 2016 (link)).

Abdurahman A., Aierken W., Zhang F., Obulkasim R., Aniwashi J, & Sulayman A. (2022). miR-1306 induces cell apoptosis by targeting BMPR1B gene in the ovine granulosa cells. Frontiers in Genetics, 13, 989912.

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Protein Quantification and Western Blotting in Granulosa Cells

Cited 1 time

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Extraction and quantification of total protein in granulosa cells was performed using conventional methods and BCA Kit (Pierce, Shanghai, China). Western blotting was performed as described previously [11 (link)]. Primary antibodies were BMPR1B-specific antibody (Abcam, ab155058, Cambridge, UK, 1:1000) and GAPDH-specific antibody (Abcam, ab9482, 1:1000), and secondary antibody was anti-goat and anti-mouse IgG (1:1000; Origene, Herford, Germany). ImageJ software was used to analyze the bands.

Abdurahman A., Du X., Yao Y., Sulaiman Y., Aniwashi J, & Li Q. (2019). Smad4 Feedback Enhances BMPR1B Transcription in Ovine Granulosa Cells. International Journal of Molecular Sciences, 20(11), 2732.

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