Pde1a

Manufactured by BPS Biosciences

Sourced in United States

PDE1A is a lab equipment product offered by BPS Biosciences. It is a recombinant human phosphodiesterase 1A enzyme. Phosphodiesterases are enzymes that catalyze the hydrolysis of cyclic nucleotides, such as cAMP and cGMP, which are important second messengers in various cellular signaling pathways.

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Lab products found in correlation

Pde4d, by BPS Biosciences (2 mentions) Pde7a, by BPS Biosciences (2 mentions) Pde3a, by BPS Biosciences (2 mentions) Pde10a, by BPS Biosciences (1 mentions) Lance ultra camp assay kit, by PerkinElmer (1 mentions) Envision multilable reader, by PerkinElmer (1 mentions) Pde4b, by BPS Biosciences (1 mentions)

2 protocols using pde1a

Enzymatic Activity Measurement of Phosphodiesterases

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The enzymatic activity of the purified catalytic domain of PDE4D, PDE5A, PDE7A, PDE9A, and PDE10A, and full-length PDE1A, PDE2A, PDE3A, PDE6C, PDE8A, and PDE11A, purchased from BPS Bioscience (San Diego, CA, USA), was performed by determining the biochemical hydrolysis of [³H]-cAMP or [³H]-cGMP into [³H]-AMP or [³H]-GMP, respectively, using the scintillation proximity assay (SPA) as described previously [25] (link). The enzymatic activities in RAW264.7 cells and skin biopsies were normalized to the concentration of total proteins.

Li H., Zhang X., Xiang C., Feng C., Fan C., Liu M., Lu H., Su H., Zhou Y., Qi Q., Xu Y, & Tang W. (2021). Identification of phosphodiesterase-4 as the therapeutic target of arctigenin in alleviating psoriatic skin inflammation. Journal of Advanced Research, 33, 241-251.

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Enzyme Inhibition Assay for PDE Enzymes

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The enzyme inhibition assay was performed against human PDE enzymes (PDE1A, PDE3A, PDE4A, PDE4B, PDE4C, PDE4D, and PDE7A; BPS Biosciences, San Diego, CA, United States) according to the manufacturer’s instructions (LANCE Ultra cAMP assay kit; Perkin Elmer, United States). In each well, 5 μl of 3 nM cAMP, 2.5 μl of PDE enzyme (0.1 ng/well), and 2.5 μl of inhibitor solution were added, and incubated at 37°C for 1 h. After incubation, 5 μL each of Eu-cAMP and ULight-anti-cAMP detection reagent supplemented with 1 mM of IBMX were added. The reaction mixture was incubated at 37°C for 1 h. After incubation, emission signals were collected at 665 nm using EnVision Multilable Reader (Perkin Elmer, United States).

Purushothaman B., Arumugam P, & Song J.M. (2018). A Novel Catecholopyrimidine Based Small Molecule PDE4B Inhibitor Suppresses Inflammatory Cytokines in Atopic Mice. Frontiers in Pharmacology, 9, 485.

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