S10-3 cells transfected with HEV RNA transcripts or infected with HEV were grown on slides, rinsed in PBS three times, and fixed with 80% acetone for 20 min. Cells were then rinsed in PBS 3 times followed by 1-h incubation with anti-HEV-ORF2 antibody (36 (link)). Next, cells were rinsed in PBS for another 3 times and treated with goat antimouse IgG conjugated with Alexa Fluor 488 (Invitrogen) for 1 h. After adding 4, 6-diamidino-2-phenylindole (DAPI) mounting solution (Sigma), cells were visualized under a fluorescence microscope (DMI3000B, Leica, Germany). HEV infectious titers were assessed by counting the number of ORF2-positive cell clusters (quantified as FFU).
6 diamidino 2 phenylindole dapi mounting solution
Manufactured by Merck Group
Sourced in Germany
6-diamidino-2-phenylindole (DAPI) mounting solution is a fluorescent dye used in the field of microscopy and cell biology. It binds to the DNA of cells, allowing researchers to visualize and identify cell nuclei. The solution is designed to be used as a mounting medium for preparing samples for microscopic examination.
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2 protocols using 6 diamidino 2 phenylindole dapi mounting solution
1
Immunofluorescence Detection of HEV Infection
2
Immunofluorescence Detection of HEV Infection
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S10-3 cells transfected with HEV RNA transcripts or infected with HEV were grown on slides, rinsed in PBS three times, and fixed with 80% acetone for 20 min. Cells were then rinsed in PBS 3 times followed by 1-h incubation with anti-HEV-ORF2 antibody (36 (link)). Next, cells were rinsed in PBS for another 3 times and treated with goat antimouse IgG conjugated with Alexa Fluor 488 (Invitrogen) for 1 h. After adding 4, 6-diamidino-2-phenylindole (DAPI) mounting solution (Sigma), cells were visualized under a fluorescence microscope (DMI3000B, Leica, Germany). HEV infectious titers were assessed by counting the number of ORF2-positive cell clusters (quantified as FFU).
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