Phenobarbitone, ketoconazole, CYP450 isoenzymes, potassium phosphate dibasic, potassium phosphate monobasic, bovine serum albumin (BSA), nicotinamide adenine dinucleotide phosphate (NADPH), ethoxyresorufin, methoxyresorufin, pentoxyresorufin, benzyloxyresorufin, dextromethorphan, diclofenac, zinc sulphate heptahydrate, trimethylamine, and acetonitrile used to evaluate the effect of xylopic acid on CYPs were all obtained from Sigma-Aldrich Chemie GmbH (Eschenstrasse), Germany. XA was purified following the procedure described by Biney et al. [24 ].
Benzyloxyresorufin
Manufactured by Merck Group
Sourced in United States, Czechia
Benzyloxyresorufin is a fluorescent dye used in biochemical research. It is a substrate for certain enzymatic assays, specifically those involving cytochrome P450 enzymes. The compound emits a red-orange fluorescent signal upon enzymatic conversion.
Automatically generated - may contain errors
Lab products found in correlation
Ethoxyresorufin, by Merck Group (6 mentions)
Methoxyresorufin, by Merck Group (5 mentions)
Resorufin, by Merck Group (5 mentions)
P nitrophenol, by Merck Group (4 mentions)
Pentoxyresorufin, by Merck Group (3 mentions)
Nadph, by Merck Group (3 mentions)
Glucose 6 phosphate (g6p), by Merck Group (3 mentions)
Glucose 6 phosphate dehydrogenase, by Merck Group (3 mentions)
Erythromycin, by Merck Group (3 mentions)
Bovine serum albumin (bsa), by Merck Group (2 mentions)
Diclofenac, by Merck Group (2 mentions)
Menadione, by Merck Group (2 mentions)
Acenaphthenol, by Merck Group (2 mentions)
Udp glucuronic acid, by Merck Group (2 mentions)
Cytochrome c, by Merck Group (2 mentions)
1 chloro 2 4 dinitrobenzene, by Merck Group (2 mentions)
Theobromine, by Merck Group (2 mentions)
Paraxanthine, by Merck Group (2 mentions)
Theophylline, by Merck Group (2 mentions)
Reduced form of β nicotinamide adenine dinu cleotide phosphate β nadph, by Oriental Yeast (2 mentions)
7 protocols using benzyloxyresorufin
1
Evaluation of Xylopic Acid's Effect on CYPs
2
Cytochrome P450 enzyme assay protocol
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Acenaphthenol, benzyloxyresorufin, bicinchoninic acid (BCA) assay kit, cytochrome c, NADH, NADPH, NADP+, 7-ethoxyresorufine, menadione, 7-methoxyresorufine, β-naphthoflavone, p-nitrophenyl sulfate, resorufin, R-sulforaphane, 3′-phosphoadenosin-5′-phosphate, and UDP-glucuronic acid were purchased from Sigma-Aldrich (Prague, Czech Republic). Midazolam was obtained from Toronto Research Chemicals (North York, ON, Canada). All other chemicals used were of HPLC or analytical grade. For real-time Polymerase Chain Reaction (PCR) analyses, ProtoScript® II Reverse Transcriptase was purchased from NEB (Ipswich, UK), TriReagent from Biotech (Prague, Czech Republic), and the qPCRCore kit for SYBR Green I from Eurogentec (Seraing, Belgium).
3
Comprehensive Assay Protocol for Hepatic Function
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DAS, thioacetamide, ethoxyresorufin, methoxyresorufin, pentoxyresorufin, benzyloxyresorufin, resorufin, p-nitrophenol, erythromycin, glucose 6-phosphate, NADPH, glucose 6-phosphate dehydrogenase and the kits for alanine aminotransferase (ALT) and aspartate aminotransferase (AST) assays were purchased from Sigma Chemical Company (St. Louis, MO, USA). The primary antibodies against individual CYP proteins were purchased from Easy-bio system (Seoul, Korea). Earle's balanced salt solution (EBSS) and guinea pig complements were purchased from GIBCO (Grand Island, NY, USA). Sheep red blood cells (SRBCs) were obtained at the College of Natural Resources at Yeungnam University. All other chemicals were of reagent grade commercially available and used as received.
4
Sesquiterpenes and Xenobiotic Metabolism
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Sesquiterpenes cis-nerolidol (CNER), trans-nerolidol (TNER), farnesol (FAR), and other chemicals benzyloxyresorufin, ethoxyresorufin, resorufin, coumarin, 7-ethoxy-4-(trifluoromethyl)coumarin, S-mephenytoin, diclofenac, bufuralol, testosterone, midazolam, chlorzoxazone, menadione, 1-chloro-2,4-dinitrobenzene, p-nitrophenol, p-nitrophenylsulphate, 2-napthol, 3’-phosphoadenosine-5’-phosphate, acenaphthenol, reduced glutathione, UDP-glucuronic acid, cytochrome c, NADH, NADP and NADPH, were purchased from Sigma-Aldrich (Prague, Czech Republic). All other chemicals used were of HPLC or analytical grade.
5
Comprehensive Metabolic Enzyme Assay
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Chrysin, caffeine, paraxanthine, theobromine, theophylline, ethoxyresorufin, methoxyresorufin, benzyloxyresorufin, resorufin, p-nitrophenol, erythromycin, 4-dimethylaminoantipyrine, glucose 6-phosphate, glucose-6-phosphate dehydrogenase, β-glucuronidase (type H-3 from Helix pomatia), sulfatase (type H-1 from Helix pomatia) and D-saccharic acid 1,4-lactone monohydrate were obtained from Sigma-Aldrich (St. Louis, MO, USA). The reduced form of β-nicotinamide adenine dinu-cleotide phosphate (β-NADPH) was purchased from Oriental Yeast Co., Ltd. (Tokyo, Japan), and methanol and acetonitrile were HPLC-grade from J.T.Baker (Center Valley, PA, USA). All other chemicals were of analytical grade and used as received.
6
Baicalin Phytochemical Analysis Protocol
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Baicalin (purity, >90.0%) was supplied form Tokyo Chemical Industry. Baicalein, caffeine, paraxanthine, theobromine, theophylline, ethoxyresorufin, methoxyresorufin, benzyloxyresorufin, resorufin, p-nitrophenol, erythromycin, glucose-6-phosphate and glucose-6-phosphate dehydrogenase were obtained from Sigma (St. Louis, MO, USA). The reduced form of β-nicotinamide adenine dinucleotide phosphate (β-NADPH) was purchased from Oriental Yeast Co., Ltd. (Tokyo, Japan), and methanol and acetonitrile were HPLC-grade from J.T. Baker (Central Valley, PA, USA). All other chemicals were of analytical grade and used as received.
7
Cytochrome P450 Activity Assays
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Bovine serum albumin (BSA), NADPH, 1-chloro-2,4-dinitrobenzene (CNDB), glutathione, o-phenylenediamine dihydrochloride (OPD), ethoxyresorufin, methoxyresorufin, benzyloxyresorufin and pentoxyresorufin, were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO). Microtiter plates (MaxiSorp) were purchased from Nunc (Roskilde, Denmark). Polyclonal CYP1A1 antibody was purchased from Biosense Laboratories, (Bergen, Norway). CYP2B6, CYP2E1 and CYP2B1/2 antibodies were purchased from Santa Cruz Biotechnology (Dallas TX, USA). The CYP3A antiserum was a kind gift from Dr. Malin Celander. All other chemicals were of the highest commercially available grade.
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