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Polyclonal rabbit anti sirt1

Manufactured by Proteintech
Sourced in United States

Polyclonal rabbit anti‐SIRT1 is a primary antibody produced in rabbits and directed against the SIRT1 protein. SIRT1 is a nicotinamide adenine dinucleotide (NAD+)-dependent deacetylase that plays a role in the regulation of various cellular processes.

Automatically generated - may contain errors

2 protocols using polyclonal rabbit anti sirt1

1

Antibody Selection for Western Blot Analysis

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Antibodies to polyclone rabbit anti‐WWP2 (1:1000; Abcam, USA; 1:500; Proteintech, USA), monoclonal rabbit anti‐HA (1:1000; Cell Signaling Technology, USA), polyclone rabbit anti‐STAT3‐K685AC (1:1000; Cell Signaling Technology, USA), polyclone rabbit anti‐STAT3‐Y705P (1:1000; Cell Signaling Technology, USA), polyclonal rabbit anti‐STAT3 (1:1000; Proteintech, USA), polyclonal rabbit anti‐SIRT1 (1:1000; Proteintech, USA), polyclonal rabbit anti‐SM22α (1:1000; Proteintech, USA), polyclonal rabbit anti‐α‐SM‐actin (1:1000; Proteintech, USA), monoclonal rabbit anti‐MMP9 (1:200; Santa, USA), polyclonal rabbit anti‐MMP2 (1:500; Proteintech, USA), polyclonal rabbit anti‐PCNA (1:1000; Proteintech, USA), polyclonal rabbit anti‐β‐Tubulin (1:2000; Proteintech, USA) and monoclonal mouse anti‐GAPDH (1:2000; Abcam, USA) were purchased commercially. Protein A/G immunoprecipitation magnetic beads were obtained from Biotool and used for immunoprecipitation.
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2

Antibody Validation for Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies to polyclone rabbit anti-Septin4 (1:1000; abcam, USA; 1:500; Proteintech, USA), monoclonal rabbit anti-Flag (1:1000; Cell Signaling Technology, USA), polyclone rabbit anti-STAT3-K685 (1:1000; Cell Signaling Technology, USA), polyclone rabbit anti-STAT3-Y705 (1:1000; Cell Signaling Technology, USA), polyclonal rabbit anti-STAT3 (1:1000; Proteintech, USA), polyclonal rabbit anti-SIRT1 (1:1000; Proteintech, USA), polyclonal rabbit anti-SM22α (1:1000; Proteintech, USA), polyclonal rabbit anti-α-SM-actin (1:1000; Proteintech, USA), monoclonal rabbit anti-MMP9 (1:200; Santa, USA), polyclonal rabbit anti-MMP2 (1:500; Proteintech, USA), polyclonal rabbit anti-PCNA (1:1000; Proteintech, USA) and monoclonal mouse anti-GAPDH (1:2000; abcam, USA) were purchased commercially. Protein A/G immunoprecipitation magnetic beads was obtained from Biotool and used for immunoprecipitation.
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