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Needle electrode

Manufactured by ADInstruments
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Sourced in Australia

The Needle Electrode is a specialized laboratory instrument designed for the accurate measurement and recording of electrical signals. It is a thin, sharp electrode that can be easily inserted into tissues or organs to detect and transmit electrical activity. The Needle Electrode is a versatile tool used in various research and clinical applications, where the precise monitoring of electrical phenomena is required.

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Lab products found in correlation

Bioelectric amplifier, by ADInstruments (3 mentions) Multiscango enzyme standard instrument, by Thermo Fisher Scientific (3 mentions) Powerlab data acquisition and analysis system, by ADInstruments (3 mentions) Imidazole, by Merck Group (2 mentions) Glutamate, by Merck Group (2 mentions) Taurine, by Merck Group (2 mentions) Atp na2, by Merck Group (2 mentions) Adp potassium salt, by Merck Group (2 mentions) Lactobionate, by Merck Group (2 mentions) Powerlab signal acquisition and analysis system, by Thermo Fisher Scientific (2 mentions) High resolution respirometry, by Oroboros (2 mentions) Na2 phosphocreatine, by Merck Group (2 mentions) Ethylene glycol tetraacetic acid (egta), by Merck Group (2 mentions) Dithiothreitol (dtt), by Merck Group (2 mentions) Hepes, by Merck Group (2 mentions) Antimycin a, by Merck Group (2 mentions) Rotenone, by Merck Group (2 mentions) Ascorbic acid, by Merck Group (2 mentions) Cytochrome c, by Merck Group (2 mentions) Malate, by Merck Group (2 mentions)

8 protocols using needle electrode

1

Oxidative Stress Biomarkers and Inflammation

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The main reagents used in the present study are listed below. ROS kits were provided by Nanjings Mr Ng biological technology Co., Ltd. The CK-MB kit was provided by Wuhan huamei biological engineering Co., Ltd. IL-6, CRP, and TNF-α kits were purchased from the Wuhan optimal, born trade Co., Ltd. TXNIP, TRX, NF-ĸ Bp65, NLRP3, and Caspase-1 antibody were obtained from Abcam Trading (Shanghai) Company Ltd. β-Actin antibody was supplied by Beijing zhongshan jinqiao biotechnology Co., Ltd.
The following main instruments were used in the present study: a PowerLab signal acquisition and analysis system, a MultiscanGO enzyme standard instrument (Thermo, United States), a Sigma 3k15 high-speed refrigerated centrifuge (SIGMA, Germany), a pressure volume catheter (SPR-838, Millar company, USA), a PowerLab data acquisition and analysis system (AD Instruments, Australia), vertical electrophoresis system (BIO-TEK, USA), transfer electrophoresis system (BIO-TEK, USA), a gel imaging system (Bio Spectrum), an image analysis system (Image-Pro Plus 4.1), a PowerLab data acquisition and analysis system (AD Instruments, Australia), a bioelectric amplifier (AD Instruments, Australia), and a needle electrode (AD Instruments, Australia).
Li Y., Xu P., Wang Y., Zhang J., Yang M., Chang Y., Zheng P., Huang H, & Cao X. (2020). Different Intensity Exercise Preconditions Affect Cardiac Function of Exhausted Rats through Regulating TXNIP/TRX/NF-ĸBp65/NLRP3 Inflammatory Pathways. Evidence-based Complementary and Alternative Medicine : eCAM, 2020, 5809298.
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2

Cardiac Biomarkers Measurement Protocols

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The main reagents used in the present study are listed below. The cardiac troponin I (cTn-I) and N terminal pro B type natriuretic peptide (NT-proBNP) enzyme-linked immunoassay kits were obtained from Cloud-Clone Corp. (Wuhan, China). ADP potassium salt, Cytochrome c, Lactobionate, ATP Na2, Na2Phosphocreatine, EGTA, Ascorbic acid, Taurine, Imidazole, DTT, HEPES, MES, Glutamate, Malate, Succinate, TMPD, Antimycin A and Rotenone were purchased from Sigma-Aldrich (St. Louis, MO, USA).
The following main instruments were used in the present study: An animal treadmill (Taimeng, China), a PowerLab signal acquisition and analysis system, a MultiscanGO enzyme standard instrument (Thermo, USA), a pressure volume catheter (SPR-838, Millar Company, USA), a PowerLab data acquisition and analysis system (AD Instruments, Australia), a bioelectric amplifier (AD Instruments, Australia), a needle electrode (AD Instruments, Australia) and a high-resolution respirometry (Oroboros Instruments, Austria).
Ping Z., Qiu W., Yang M., Zhang X., Wang D., Xu P., Wang Z, & Cao X. (2021). Optimization of different intensities of exercise preconditioning in protecting exhausted exercise induced heart injury in rats. Sports Medicine and Health Science, 3(4), 218-227.
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3

Cardiac Biomarker Detection Assay

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The main reagents used in the present study are listed below. The cardiac troponin I (cTnI) and N-terminal pro B type natriuretic peptide (NT-proBNP) enzyme-linked immunoassay kits were obtained from Shanghai Kmaels Biologic Technology Co., Ltd., ADP potassium salt, cytochrome c, lactobionate, ATP-Na2, Na2Phosphocreatine, EGTA, ascorbic acid, taurine, imidazole, DTT, HEPES, MES, glutamate, malate, succinate, TMPD, antimycin A and rotenone were purchased from Sigma (USA). The following main instruments were used in the present study: an animal treadmill (Taimeng, China), a PowerLab signal acquisition and analysis system, a MultiscanGO enzyme standard instrument (Thermo, USA), a pressure volume catheter (SPR-838, Millar Company, USA), a PowerLab data acquisition and analysis system (AD Instruments, Australia), a bioelectric amplifier (AD Instruments, Australia), a needle electrode (AD Instruments, Australia) and a high-resolution respirometry (Oroboros Instruments, Austria).
Su Y., Wang Y., Xu P., Sun Y., Ping Z., Huang H, & Cao X. (2021). Study on the time-effectiveness of exercise preconditioning on heart protection in exhausted rats. The Chinese journal of physiology, 64(2).
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4

Multimodal Cardiovascular Monitoring

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Electrocardiography (needle electrode, ADInstruments, Dunedin, New Zealand) was recorded continuously, and systolic blood pressure (Non‐Invasive Blood Pressure, ADInstruments) monitored intermittently (5–10 min) via cuff inflation on the forelimb. Microvascular perfusion was continuously measured over proximal (interscapular) and distal (dorsal ear) skin sites using laser Doppler flowmetry (LDF, Probe 457, PeriFlux 5001, Perimed, Jarfalla, Sweden) affixed to depillated skin sites using modified TCO2 fixation rings and double sided tape.
Sixtus R.P., Gray C., Berry M.J, & Dyson R.M. (2021). Nitrous oxide improves cardiovascular, respiratory, and thermal stability during prolonged isoflurane anesthesia in juvenile guinea pigs. Pharmacology Research & Perspectives, 9(1), e00713.
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5

Cardiac Tissue Action Potential Recording

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The bioreactor was opened, and two needle electrodes (29G in diameter; ADInstruments, New South Wales, Australia) were placed over the cardiac tissue. Surface action potentials were amplified (UA102 amplifier, Unique Medical, Tokyo, Japan) and recorded using a data acquisition system (ML870 PowerLab 8/30; ADInstruments).
Inui A., Sekine H., Sano K., Dobashi I., Yoshida A., Matsuura K., Kobayashi E., Ono M, & Shimizu T. (2019). Generation of a large-scale vascular bed for the in vitro creation of three-dimensional cardiac tissue. Regenerative Therapy, 11, 316-323.
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6

Surface ECG in Anesthetized Mice

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For surface electrocardiography, mice were anesthetized with 1.75% isoflurane at a core temperature of 37°C. Needle electrodes (AD Instruments) were placed subcutaneously in standard limb lead configurations. Signals were sampled using a PowerLab16/30 interface (AD Instruments). Data analysis was performed using LabChart (v 7.3.7, AD Instruments).
Agudo J., Ruzo A., Park E.S., Sweeney R., Kana V., Wu M., Zhao Y., Egli D., Merad M, & Brown B.D. (2015). JEDI T-cells enable targeted cell depletion and investigation of T-cell interactions with virtually any cell population. Nature biotechnology, 33(12), 1287-1292.
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7

Surface ECG in Anesthetized Mice

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For surface electrocardiography, mice were anesthetized with 1.75% isoflurane at a core temperature of 37°C. Needle electrodes (AD Instruments) were placed subcutaneously in standard limb lead configurations. Signals were sampled using a PowerLab16/30 interface (AD Instruments). Data analysis was performed using LabChart (v 7.3.7, AD Instruments).
Agudo J., Ruzo A., Park E.S., Sweeney R., Kana V., Wu M., Zhao Y., Egli D., Merad M, & Brown B.D. (2015). JEDI T-cells enable targeted cell depletion and investigation of T-cell interactions with virtually any cell population. Nature biotechnology, 33(12), 1287-1292.
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8

Visceral Pain Assessment via VMR

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For assessing the visceral pain, the visceromotor response (VMR) to colorectal distention (CRD) was measured using electromyography (EMG), as described previously.9 Briefly, under isoflurane anesthesia, a latex balloon (Okamoto, Tokyo, Japan) was inserted into the distal colon, and needle electrodes (AD instruments, Bella Vista, Australia) were placed on the right external oblique musculature for obtaining EMG recordings. Next, rats were anesthetized with 0.5% propofol (5 mL/h for 5 min and 6–7.5 mL/h/kg continuous intravenous administration), and their EMG signals were recorded after recovery from isoflurane anesthesia (approximately 30 min) using a data acquisition system equipped with a software (PowerLab 4/25, Chart, AD instruments). After evaluating the visceral pain, CRD was performed thrice by tonic inflation up to 60 mmHg over a period of 10 s with an interval of 5 min. VMR to CRD was quantified as the maximal value of area under the curve (AUC) of EMG for 5 s.
Kogure Y., Kanda H., Wang S., Hao Y., Li J., Yamamoto S., Noguchi K, & Dai Y. (2020). Daikenchuto attenuates visceral pain and suppresses eosinophil infiltration in inflammatory bowel disease in murine models. JGH Open: An Open Access Journal of Gastroenterology and Hepatology, 4(6), 1146-1154.
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