AP20187 (MedChemExpress, HY-13992) was resuspended in 75% EtOH at 62.5 mg/ml and stored at −20°C. For in vitro experiments, cells were treated with 100 nM AP20187 for 48–72 h. In vivo, the compound was dissolved in 2% Tween-20 (Sigma-Aldrich, P7949) and 10% PEG-300 (Sigma-Aldrich #202371) and mice were i.p. injected with 2.5 mg/kg AP20187.
Ap20187
Manufactured by Merck Group
AP20187 is a laboratory equipment product manufactured by Merck Group. It is designed to perform specific functions in a research or analytical setting. The core function of this product is to enable users to conduct experiments or analyses, but further details about its intended use are not provided to maintain an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
Lsm 880, by Zeiss (2 mentions)
Sp6 rna polymerase, by Thermo Fisher Scientific (1 mentions)
Mtt assay kit, by Promega (1 mentions)
Su5416, by Merck Group (1 mentions)
Ap20187, by Takara Bio (1 mentions)
Alexa fluor plus 405 phalloidin, by Thermo Fisher Scientific (1 mentions)
Airyscan processing, by Zeiss (1 mentions)
P7949, by Merck Group (1 mentions)
Peg300, by Merck Group (1 mentions)
Ap20187, by MedChemExpress (1 mentions)
4 protocols using ap20187
1
Resuspension and Application of AP20187 Compound
2
Induction and Rescue of LR Asymmetry
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iXFGFR4-pCS2+ (Addgene 31258) and iXFGFR1-pCS2+ (Addgene 31257)55 (link) were linearized with NotI restriction enzyme and in vitro transcribed with SP6 RNA polymerase (Invitrogen AM1340) to produce ifgfr4 and ifgfr1 mRNAs for microinjection. For iFgfr4 induction during leftward flow stages, 20 nl of 1 μM AP20187 (Sigma SML2838) was injected into the gastrocoel cavity of St. 15 embryos. For rescue of rspo2 derived LR asymmetry defects, embryos co-injected with rspo2 and ifgfr mRNAs were transferred to 0.1x MR (Modified Ringer’s) solution supplemented with 1 μM AP20187 at St. 10. Embryos were rinsed carefully and transferred to 0.1x MR solution without AP20187 at St. 12-13 and harvested for in situ hybridization.
3
Cell Viability Assay for Angiogenesis Inhibitors
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Cells were plated 1 × 104 per well in 96-well microtiter plates and incubated in DMEM with 10% FBS for 24 h. Then, either AP20187, SU5416 (Sigma-Aldrich), VEGF-A neutralizing antibody (NAB, R&D Systems, Minneapolis, MN), or vehicle (either ethanol, DMSO, or PBS with control mouse IgG) was added to the media and the cultures were incubated for additional 16 h. Cell viability was determined by the MTT Assay Kit (Promega, Madison, WI) according to the manufacturer's instructions.
4
Visualizing Mechanosensory Structures in Vestibular Hair Cells
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Explant cultures of vestibular sensory epithelia were fixed in 4% paraformaldehyde (PFA; Electron Microscopy Sciences) in PBS for 30 min at room temperature (RT) and washed in PBS. For conditional dimerization, samples were treated with 100 nM AP20187 (Sigma) or 500 nM AP21987 (Takara Bio) for 2 h. The concentration of AP20187 was lowered because 200 nM AP21087 caused strong accumulation of MYO7A-HMM dimers at stereocilia tips and often damaged the architecture of stereocilia. Samples were permeabilized and blocked in PBS containing 1% bovine serum albumin (BSA) and 0.2% Triton-X100 for 20–30 min at RT. HaloTag-fused proteins were visualized by reacting with 200 nM JFX554-HaloTag-ligand52 (link) (gift from Luke Lavis) in PBS with 0.2% Triton-X 100 for 1 h at RT. F-actin was visualized by supplementing the HaloTag ligand solution with 10–30 nM of Alexa Fluor™ Plus 405 Phalloidin (Thermo Fisher). Confocal images were acquired using a Zeiss LSM880 with Airyscan processing (Zeiss).
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