Perfluorononanoic acid

The 21 chemicals [sodium (meta) arsenite, cadmium chloride, copper (II) chloride, lead (II) acetate trihydrate, mercury (II) chloride, phenanthrene, p,p′-DDE (dichlorodiphenyldichloroethylene), thiram, 1,4-dichlorobenzene, 2,5-dichlorophenol, methylparaben, propylparaben, perfluorodecanoic acid (PFDA), perfluorononanoic acid (PFNA), perfluorooctanoic acid (PFOA), perfluorooctanesulfonic acid (PFOS), polychlorinated biphenyl 153 (PCB153), polychlorinated biphenyl 187 (PCB187), bisphenol A, bisphenol F, bisphenol S] were purchased from Sigma-Aldrich and Cayman Chemicals (Table S1). The chemicals were weighed and dissolved in dimethyl sulfoxide (DMSO) (for most) and in sterile water (for some) at a concentration of $5\mathrm{mg}/\mathrm{mL}$ and stored at $-20°\mathrm{C}$ for long-term storage. For dosing, we prepared an intermediate stock concentration of $5\text{\hspace{0.17em}mM}$ for each of the chemicals, which was further diluted into final concentrations for dosing the MCF10A cells: $25\mathrm{\mu }\mathrm{M}$ , $2.5\mathrm{\mu }\mathrm{M}$ , $0.25\mathrm{\mu }\mathrm{M}$ , and $0.025\mathrm{\mu }\mathrm{M}$ . These final concentrations were prepared fresh in MCF10A media for each experiment. For chemicals dissolved in DMSO, the final DMSO concentration was 0.5%, as were the vehicle controls. For chemicals dissolved in water, an equivalent amount of water was included in the vehicle control.