Unless specified, all reagents were obtained from Sigma and all the antibodies were from Santa Cruz Biotechnology, except for anti-HIF-1α (Becton Dickinson), anti-PKM2 and anti-Src-Tyr416 (Cell Signaling Technology) and anti phospho-Tyr (clone 4G10) (Millipore). HRP-conjugated streptavidin was from Pierce. HIF-1-siRNA and DEC1-siRNA were from Santa Cruz Biotechnology. N-(biotinoyl)-N-(iodoacetyl)ethylenediamine (BIAM) and 2′-7′-dichlorofluoresceindiacetate (H2-DCF-DA) were from Molecular Probe. [U-14C] lactate and [U-14C] glucose were from Perkin Elmer. All the kits used to perform miRNA extraction and quantitative reverse transcriptase PCR were from Qiagen. Lipofectamine 2000 was from Invitrogen. Metformin was obtained by Sigma. The chemical synthesis of the PKM2 activator DASA-58 was kindly performed by Dr. Richichi of the Department of Chemistry (University of Florence). The mitochondrial antioxidant MitoTEMPO was from Santa Cruz Biotechnology. The MCT1 inhibitor, AR-C155858, was from Tocris Bioscience.
N biotinoyl n iodoacetyl ethylenediamine biam
Manufactured by Thermo Fisher Scientific
N-(biotinoyl)-N-(iodoacetyl)ethylenediamine (BIAM) is a biotinylated reagent used in biochemical applications. It functions as a thiol-reactive probe that can be utilized for the detection and analysis of sulfhydryl-containing proteins.
Automatically generated - may contain errors
Lab products found in correlation
Enhanced chemiluminescence ecl immunoblot detection reagents, by Thermo Fisher Scientific (2 mentions)
Aprotinin, by Merck Group (2 mentions)
Leupeptin, by Merck Group (2 mentions)
Bestatin, by Merck Group (2 mentions)
Ar c155858, by Bio-Techne (1 mentions)
U 14c glucose, by PerkinElmer (1 mentions)
Mito tempo, by Santa Cruz Biotechnology (1 mentions)
Hif 1 sirna, by Santa Cruz Biotechnology (1 mentions)
Anti hif 1α, by BD (1 mentions)
2 7 dichlorofluorescein diacetate h2dcfda, by Thermo Fisher Scientific (1 mentions)
U 14c lactate, by PerkinElmer (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Metformin, by Merck Group (1 mentions)
Sec498cys, by AbFrontier (1 mentions)
Horseradish peroxidase hrp conjugated streptavidin, by GE Healthcare (1 mentions)
Ethylene diamine tetraacetic acid (edta), by Merck Group (1 mentions)
Insulin, by Merck Group (1 mentions)
Protease inhibitor cocktail, by Merck Group (1 mentions)
Phosphatase inhibitor, by Merck Group (1 mentions)
Nadph, by Merck Group (1 mentions)
3 protocols using n biotinoyl n iodoacetyl ethylenediamine biam
1
Metabolic Regulation in Cancer Cells
2
Enzymatic Assays for Oxidative Stress
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Purified rat liver TrxR1, recombinant
human Trx1, and glutathione reductase (GR), recombinant E.
coli Trx1, bovine glutathione peroxidase (GPx), insulin,
NADPH, 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB), APAP, NAPQI,
menadione (2-methyl-1,4-napthoquinone), reduced glutathione (GSH),
oxidized glutathione (GSSG), phosphatase inhibitors (catalog no. P2850,
which contains microcystinLR, cantharidin, and (−)-p-bromotetramisole), and protease inhibitor cocktail (catalog
no. P2714, which contains 4-(2-aminoethyl)benzenesulfonyl fluoride,
E-64, bestatin, leupeptin, aprotinin, and EDTA) were purchased from
Sigma-Aldrich (St. Louis, MO). Sodium aurothiomalate hydrate was from
Aldrich (Milwaukee, WI). Human recombinant TrxR mutant enzyme (Sec498Cys)
was from AbFrontier (Seoul, Korea). Pooled human liver microsomes
(HLM) and recombinant human P450s 1A2, 2E1, and 3A4 were purchased
from BD Genetest (Woburn, MA). N-(Biotinoyl)-N′-(iodoacetyl) ethylenediamine (BIAM) was from Molecular
Probes (Eugene, OR) and horseradish peroxidase (HRP)-conjugated streptavidin
from GE Healthcare (Piscataway, NJ). Enhanced chemiluminescence (ECL)
immunoblot detection reagents were from Thermo Scientific (Rockford,
IL).
human Trx1, and glutathione reductase (GR), recombinant E.
coli Trx1, bovine glutathione peroxidase (GPx), insulin,
NADPH, 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB), APAP, NAPQI,
menadione (2-methyl-1,4-napthoquinone), reduced glutathione (GSH),
oxidized glutathione (GSSG), phosphatase inhibitors (catalog no. P2850,
which contains microcystinLR, cantharidin, and (−)-p-bromotetramisole), and protease inhibitor cocktail (catalog
no. P2714, which contains 4-(2-aminoethyl)benzenesulfonyl fluoride,
E-64, bestatin, leupeptin, aprotinin, and EDTA) were purchased from
Sigma-Aldrich (St. Louis, MO). Sodium aurothiomalate hydrate was from
Aldrich (Milwaukee, WI). Human recombinant TrxR mutant enzyme (Sec498Cys)
was from AbFrontier (Seoul, Korea). Pooled human liver microsomes
(HLM) and recombinant human P450s 1A2, 2E1, and 3A4 were purchased
from BD Genetest (Woburn, MA). N-(Biotinoyl)-N′-(iodoacetyl) ethylenediamine (BIAM) was from Molecular
Probes (Eugene, OR) and horseradish peroxidase (HRP)-conjugated streptavidin
from GE Healthcare (Piscataway, NJ). Enhanced chemiluminescence (ECL)
immunoblot detection reagents were from Thermo Scientific (Rockford,
IL).
3
Enzyme Kinetics of Redox Regulators
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