On postnatal day 2 all pups were randomly assigned to receive treatment with either flutamide, a selective antagonist of the androgen receptor (Sigma Aldrich, St. Louis, MO) or vehicle (Veh), sesame oil (Fisher Scientific, Waltham, MA). On postnatal days 2, 4, and 6, pups were given 250mg subcutaneous injections (volume 0.1mL) on their dorsum. Injection sites were sealed using Vetbond Surgical Adhesive (3M, Maplewood, MN).
Sesame oil
Manufactured by Thermo Fisher Scientific
Sourced in United States
Sesame oil is a laboratory-grade oil used as a medium for preparing samples or reagents in various scientific experiments and analyses. It is a clear, odorless liquid with a neutral pH. Sesame oil is often used as a diluent, lubricant, or solvent in various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Human chorionic gonadotropin (hcg), by Merck Group (2 mentions)
17β estradiol e2, by Merck Group (2 mentions)
Equine chorionic gonadotropin, by Merck Group (2 mentions)
Flutamide, by Merck Group (1 mentions)
Brdu staining kit, by Thermo Fisher Scientific (1 mentions)
5 bromo 2 deoxyuridine brdu, by Thermo Fisher Scientific (1 mentions)
In situ cell death detection kit, by Roche (1 mentions)
M2 medium, by Merck Group (1 mentions)
8 protocols using sesame oil
1
Postnatal Androgen Receptor Antagonist Treatment
2
Perinatal Testosterone Exposure in Rats
3
Hormone-Induced Uterine Responses in Dgcr8 Mice
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Eight-week-old Dgcr8f/f and Dgcr8d/d mice were ovariectomized, rested for 2 weeks, and treated subcutaneously with either 0.1 ml sesame oil as vehicle (Acros, NJ, USA), 200 ng E2 (Sigma-Aldrich), or 200 ng E2 + 2 mg P4 (Sigma-Aldrich). Mice were sacrificed 24 h after steroid hormone treatment(s). 5-Bromo-2´-Deoxyuridine (BrdU) (Invitrogen Life Technologies) was given to the mice 3 h before they were sacrificed. Uteri were dissected, fixed in 4% paraformaldehyde and then subjected to paraffin-embedded tissue processing. Uterine sections were utilized for BrdU immunostaining using a BrdU staining kit (Invitrogen Life Technologies), according to the manufacturer’s instructions.
Uterine apoptosis of Dgcr8d/d mice was assessed using an In Situ Cell Death Detection Kit according to the manufacturer instructions (Roche, West Sussex, UK). Sections were deparaffinized and rehydrated in a graded alcohol series, and then processed for antigen retrieval. They were incubated with TUNEL reaction mixture for 1 h at 37 °C and then with DAPI for 10 min at room temperature, and observed under a fluorescence microscope.
Uterine apoptosis of Dgcr8d/d mice was assessed using an In Situ Cell Death Detection Kit according to the manufacturer instructions (Roche, West Sussex, UK). Sections were deparaffinized and rehydrated in a graded alcohol series, and then processed for antigen retrieval. They were incubated with TUNEL reaction mixture for 1 h at 37 °C and then with DAPI for 10 min at room temperature, and observed under a fluorescence microscope.
4
Estrogen Regulation of CREBZF in Uteri
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Three-week old and OVX female ICR mice were injected subcutaneously with
17β-estradiol (E2, 10 ug/kg, Sigma-Aldrich, USA) and Sesame oil (100 uL,
Acros, USA) as control group. The Uteri were collected at 0, 1, 3, 6, 12, and 24
hours after injection of hormones from sacrificed mice. To identify whether the
expression of CREBZF is regulated by E2 administration, an estrogen receptor
antagonist (ICI 182,780, 25 mg/kg) was co-injected to OVX mice.
17β-estradiol (E2, 10 ug/kg, Sigma-Aldrich, USA) and Sesame oil (100 uL,
Acros, USA) as control group. The Uteri were collected at 0, 1, 3, 6, 12, and 24
hours after injection of hormones from sacrificed mice. To identify whether the
expression of CREBZF is regulated by E2 administration, an estrogen receptor
antagonist (ICI 182,780, 25 mg/kg) was co-injected to OVX mice.
5
Estrogen-Induced Allergic Airway Model
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From day –7 to 20, OVX-OVA-E2 group animals were subcutaneously given E2 (Sigma–Aldrich, St. Louis, MO, U.S.A.) at 30 μg/kg/day for 4 weeks. The pulverized E2 was dissolved in absolute alcohol to the concentration of 50 μg/ml, and then diluted in sesame oil (Acros) to the concentration of 5 μg/ml. The OVX-OVA group was subcutaneously injected with the same volume of sesame oil as placebo simultaneously [26 (link)].
Twenty-four hours after the last challenge on day 21, the mice were killed. Then serum samples were collected, and the bronchoalveolar lavage (BAL) fluid (BALF) was collected from the left lung. The right lung was appropriately disposed for histopathological, immunofluorescence staining analysis, real-time RT-PCR (Q-PCR), and Western blot assays.
Twenty-four hours after the last challenge on day 21, the mice were killed. Then serum samples were collected, and the bronchoalveolar lavage (BAL) fluid (BALF) was collected from the left lung. The right lung was appropriately disposed for histopathological, immunofluorescence staining analysis, real-time RT-PCR (Q-PCR), and Western blot assays.
6
Estradiol Hormone Preparation Protocol
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17β-estradiol (E2) (Sigma-Aldrich, St. Louis, MO, USA) was dissolved in sesame oil (Acros Organics). Equine chorionic gonadotropin (eCG) and human chorionic gonadotropin (hCG) were purchased from Sigma-Aldrich.
7
Time-dependent Regulation of Cfp1 by E2 and P4
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To investigate time-dependent actions of E2 or P4 on the expression of Cfp1 in C57BL/6 mice uterus, adult (8–10 weeks of age) female mice were OVX, rested for 14 days, and then subcutaneously injected with either vehicle (sesame oil, 0.1 mL/mouse; Acros, NJ, USA), E2 (100 ng/mouse, Sigma–Aldrich, St. Louis, MO, USA) or E2 + P4 (2 mg/mouse, Sigma–Aldrich). After hormone injection, the mice were sacrificed at various time points (0–24 h) and the uterus was collected for real-time RT-PCR (n = 4 to 6 per each group).
8
Pregnancy Examination in Tsg101 Mice
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17β-estradiol (E 2 ) (Sigma-Aldrich, St. Louis, MO, USA) was dissolved in sesame oil (Acros Organics).
Equine chorionic gonadotropin (eCG) and human chorionic gonadotropin (hCG) were purchased from Sigma-Aldrich. Examination of mice on days 4 and 6 of pregnancy Tsg101 f/f and Tsg101 d/d female mice (9 to 13-week-old) received 2.5 IU of eCG and hCG at 48 h intervals to promote mating. Immediately after hCG injection, they were bred with stud male mice. On the following morning, the formation of a vaginal plug was con rmed, and females with plugs were considered to be on day 1 of pregnancy. To examine implantation sites on day 6 of pregnancy, mice received a blue dye injection (1% Chicago blue B in phosphate buffered saline (PBS; Gibco, Thermo Fisher Scienti c, Waltham, MA, USA) and sacri ced 3 min later. When no implantation site was visible, uteri were ushed with M2 medium (M7167, Sigma-Aldrich). Some mice were sacri ced at 11 AM on day 4 of pregnancy to con rm the presence of embryos. One uterine horn was ushed with M2 media and the other was processed for histological analyses.
Equine chorionic gonadotropin (eCG) and human chorionic gonadotropin (hCG) were purchased from Sigma-Aldrich. Examination of mice on days 4 and 6 of pregnancy Tsg101 f/f and Tsg101 d/d female mice (9 to 13-week-old) received 2.5 IU of eCG and hCG at 48 h intervals to promote mating. Immediately after hCG injection, they were bred with stud male mice. On the following morning, the formation of a vaginal plug was con rmed, and females with plugs were considered to be on day 1 of pregnancy. To examine implantation sites on day 6 of pregnancy, mice received a blue dye injection (1% Chicago blue B in phosphate buffered saline (PBS; Gibco, Thermo Fisher Scienti c, Waltham, MA, USA) and sacri ced 3 min later. When no implantation site was visible, uteri were ushed with M2 medium (M7167, Sigma-Aldrich). Some mice were sacri ced at 11 AM on day 4 of pregnancy to con rm the presence of embryos. One uterine horn was ushed with M2 media and the other was processed for histological analyses.
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