Anti-Flag (#F1804) and anti-vinculin (#V9131) antibodies were from Sigma-Aldrich (St. Louis, MO, USA). Phos-tag acrylamide (#AAL-107) was from Wako Chemicals (Richmond, VA, USA). BX795 (#14932) was from Cayman (Ann Arbor, MI, USA). Rapamycin (#5318893) was from Peprotech (East Windsor, NJ, USA).
Bx795
Manufactured by Cayman Chemical
Sourced in United States
BX795 is a laboratory reagent manufactured by Cayman Chemical. It is a small molecule that functions as a potent and selective inhibitor of PDK1, a key kinase in the PI3K/Akt signaling pathway. The product is intended for research use only.
Automatically generated - may contain errors
Lab products found in correlation
Anti vinculin, by Merck Group (2 mentions)
Anti flag, by Merck Group (2 mentions)
Phos tag acrylamide, by Fujifilm (2 mentions)
Rapamycin, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Mem amino acids, by Thermo Fisher Scientific (1 mentions)
Llome, by Cayman Chemical (1 mentions)
α sma antibody, by Abcam (1 mentions)
Epinephrine, by Merck Group (1 mentions)
Forskolin, by Cayman Chemical (1 mentions)
Lats1, by Cell Signaling Technology (1 mentions)
Lamin a c, by Cell Signaling Technology (1 mentions)
3 protocols using bx795
1
Antibody and Reagent Sourcing
2
Amino Acid Stress Response Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HeLa (kindly provided by Pietro De Camilli, Yale University) and RAW 264.7 (ATCC) cells were maintained in DMEM + 10 % FBS + 1 % penicillin/streptomycin (Thermo Fisher Scientific) at 37 °C with 5 % CO2. Details of cell lines are summarized in Supplemental Table 1 . Experiments to test the effect of amino acids on cells were performed as per our previous studies (Amick et al., 2016 (link); Amick et al., 2020 ; Meng and Ferguson, 2018 (link); Petit et al., 2013 (link)). In brief, cells were washed 2 X in PBS and incubated in RPMI without amino acids (US Biological) for 1 h and then were shifted to RPMI with MEM Amino Acids (Gibco) at the indicated times. BX-795 (Cayman, dissolved in DMSO) and LLOMe (Cayman, dissolved in DMSO) were added to growing cells at the indicated concentrations and compared with cells treated with an equivalent amount of DMSO alone. Details of growth media and drugs are summarized in Supp. Table 2 .
3
Antibodies and Small Molecules Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Anti-Flag (#F1804) and anti-Vinculin (#V9131) antibodies were from Sigma. Antibodies against IRF3 (#sc-33641), p-CREB1 (#sc-81,486), IRF7 (#sc-74471), Vimentin (#sc-32322) were obtained from Santa Cruz. α-SMA antibody were from Abcam (#ab7817). IL-33 (AF3626) antibody was from R&D systems. LATS1 (#3477), HA-tag (#2367) and Lamin A/C (#2032) antibodies were from Cell Signaling. Epinephrine (#E4250) was from Sigma. Phos-tag acrylamide (#AAL-107) was from Wako Chemicals. Glucagon (#24204), Forskolin (#11018) and BX795 (#14932) were from Cayman. H89 (#S1582) was from Selleckchem.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!