Total RNA was isolated from human placenta using Trizol (Invitrogen, USA) according to the manufacturer’s directions. RNA quality was determined using Agilent 6000 Nano chips on an Agilent Technologies 2100 Bioanalyzer. Samples with RIN > 6 were selected for library construction. Sequencing libraries were constructed in a single batch according to the TruSeq SmallRNA Libary Preparation guide (Illumina) with no modification. In each sequencing lane on an Illumina HiSeq X10 platform, we pooled 14 samples with different index sequences and carried out 150-bp paired-ended sequences. We randomly distributed 10 African American, 10 European American, 10 South Asian American, and nine East Asian American samples into three sequencing lanes. We further included the tenth East Asian American sample (X14) into each of the three lanes to control for potential sequencing artifacts among the lanes (Additional file 1 : Table S1).
Truseq smallrna libary preparation guide
Manufactured by Illumina
The TruSeq SmallRNA Library Preparation Guide is a laboratory tool designed for the preparation of small RNA libraries. It provides a standardized protocol and necessary reagents for the conversion of small RNA molecules into sequencing-ready libraries. The guide outlines the steps required to isolate, purify, and convert small RNA samples into adapter-ligated libraries suitable for next-generation sequencing analysis.
Automatically generated - may contain errors
2 protocols using truseq smallrna libary preparation guide
1
Transcriptome Profiling of Placental RNA
2
RNA-seq of Human Placenta Transcriptome
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total RNA was isolated from human placenta using the Trizol (Invitrogen, USA) according to the manufacturer's directions. RNA quality was determined using Agilent 6000 Nano chip on Agilent Technologies 2100 Bioanalyzer. Samples with RIN > 6 were selected for library construction. Sequencing libraries were constructed in a single batch according to the TruSeq SmallRNA Libary Preparation guide (Illumina) with no modi cation. In each sequencing lane on an Illumina HiSeq X10 platform, we pooled 14 samples with different index sequences and carried out 150-bp paired-ended sequences. In total, 42 samples with two technical replicates were sequenced on three lanes.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!