Four deoxynucleotide triphosphates dntps

Manufactured by New England Biolabs

The four deoxynucleotide triphosphates (dNTPs) are the fundamental building blocks for DNA synthesis. They include dATP, dTTP, dCTP, and dGTP.

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Lab products found in correlation

Ab5823, by Abcam (1 mentions) Ab17339, by Abcam (1 mentions) Ab135940, by Abcam (1 mentions) M2 beads, by Merck Group (1 mentions) Ap0063, by Bioworld Technology (1 mentions) Goat anti rabbit igg hrp sc 2004, by Santa Cruz Biotechnology (1 mentions) Pol β specific antibody ab26343, by Abcam (1 mentions) Goat anti mouse igg hrp sc 2005, by Santa Cruz Biotechnology (1 mentions)

2 protocols using four deoxynucleotide triphosphates dntps

Histone Peptide Modification Assay

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The histone peptides were purchased from Chinese Peptide Company, all the primers and DNA substrates used in this paper was synthesized by GenScript, Inc. Four deoxynucleotide triphosphates (dNTPs) were purchased from New England Biolabs (N0446S). [γ-³²P]-ATP (BLU002A, 250 μCi) and [α-³²P]-dCTP (NEG513H, 250 μCi) were purchased from PerkinElmer. The M2 beads and M-280 beads were purchased from Sigma-Aldrich. The DNA Ligase I was purchased from Abnova. The antibodies used in this paper are as follow: anti-FEN1 (GTX70185, Genetex), anti-PRMT5 (sc-376937, Santa Cruz), anti-8-oxoG (sc-130914, Santa Cruz), anti-H4 (PTM-1003, PTM Bio), anti-H4R3me1 (ab17339, Abcam), anti-H4R3me2s (ab5823, Abcam), anti-OGG1 (ab135940, Abcam), anti-Flag (AP0007MH, Bioword), anti-GAPDH (AP0063, Bioworld), anti-Tubulin (AM031A, Abgent).

Ma Z., Wang W., Wang S., Zhao X., Ma Y., Wu C., Hu Z., He L., Pan F, & Guo Z. (2020). Symmetrical dimethylation of H4R3: A bridge linking DNA damage and repair upon oxidative stress. Redox Biology, 37, 101653.

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Biochemical Assays for DNA Repair Proteins

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All primers and DNA substrates used in this paper were synthesized by GenScript, Inc. using polyacrylamide gel electrophoresis (PAGE) purification. Four deoxynucleotide triphosphates (dNTPs) were purchased from New England Biolabs (N0446S). [γ-³²P]-ATP (BLU002A, 250 uCi) and [α-³²P]-dCTP (NEG513H, 250uCi) were purchased from PerkinElmer. The Pol β-specific antibody (ab26343) was purchased from Abcam. The anti-PCNA (SC-7907), APE1 (SC-55498), FEN1 (SC-56675), c-Myc (SC-40), Tubulin (sc-23950), goat anti-rabbit IgG-HRP (SC-2004), and goat anti-mouse IgG-HRP (sc-2005) antibodies were purchased from Santa Cruz Biotechnology.

Zhou T., Pan F., Cao Y., Han Y., Zhao J., Sun H., Zhou X., Wu X., He L., Hu Z., Chen H., Shen B, & Guo Z. (2016). R152C DNA Pol β mutation impairs base excision repair and induces cellular transformation. Oncotarget, 7(6), 6902-6915.

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