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L glutamine and sodium pyruvate

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L-glutamine and sodium pyruvate are laboratory reagents commonly used in cell culture and biochemical applications. L-glutamine is an amino acid that serves as a primary energy source for many cell types. Sodium pyruvate is a salt of pyruvic acid, which is an important intermediate in cellular metabolism. These reagents are used to supplement cell culture media to support the growth and metabolism of various cell lines.

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Lab products found in correlation

Hygromycin b, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Atlanta Biologicals (2 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Antibiotic antimycotic, by Thermo Fisher Scientific (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Poly adp ribose polymerase parp, by Cell Signaling Technology (1 mentions) Mouse monoclonal β actin antibody, by Merck Group (1 mentions) Crude fucoidan from f vesiculosus, by Merck Group (1 mentions) Cleaved parp d214, by Cell Signaling Technology (1 mentions) 4.5 g l glucose, by Thermo Fisher Scientific (1 mentions) Phospho beclin 1 ser15, by Cell Signaling Technology (1 mentions) Anti rabbit igg hrp linked antibody, by Cell Signaling Technology (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Trypsin edta, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Sf9 cells, by Thermo Fisher Scientific (1 mentions) Glutamax, by Thermo Fisher Scientific (1 mentions) Hek293t cell, by American Type Culture Collection (1 mentions) Geneticin, by Thermo Fisher Scientific (1 mentions) Pen strep, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

L-glutamine (14671 citations) Sodium pyruvate (4375 citations) Glutamine (2902 citations) Pyruvate (379 citations) L-glutamine and pyruvate (3 citations) L-glutamine sodium pyruvate (2 citations) Glutamine and sodium pyruvate (2 citations)

7 protocols using l glutamine and sodium pyruvate

1

Stable Cell Lines for GPCR Signaling

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Human embryonic kidney (HEK) cells stably expressing AC1, AC8, or AC1 with the MOR were cultured in high-glucose (4500 mg/L) Dulbecco’s Modified Eagle Medium containing L-glutamine and sodium pyruvate (Life Technologies, Grand Island, NY) supplemented with 5% bovine calf serum (Hyclone, Logan, UT), 5% fetal clone I (Hyclone), Antibiotic-Antimycotic (Life Technologies), and G418 (Invivogen, San Diego, CA) (HEK-AC1), or hygromycin B (Fisher Scientific, Pittsburg, PA) (HEK-AC8), or G418 and puromycin (Sigma-Aldrich) (HEK-AC1/MOR). Chinese hamster ovary (CHO) cells expressing the MOR (CHO-MOR) in the PathHunter® β-Arrestin GPCR assay platform were purchased from DiscoveRx (Freemont, CA). Cells were grown in Ham’s F12 media supplemented with 1 mM L-glutamine (Thermo Scientific, West Palm Beach – FL), 10% fetal bovine serum (Hyclone), 50 U/ml penicillin, 50 µg/ml streptomycin (Life Technologies), G418 and hygromycin B. Cells were grown and frozen as previously described (53 (link)).
Brust T.F., Alongkronrusmee D., Soto-Velasquez M., Baldwin T.A., Ye Z., Dai M., Dessauer C.W., van Rijn R.M, & Watts V.J. (2017). Identification of a selective small molecule inhibitor of type 1 adenylyl cyclase activity with analgesic properties. Science signaling, 10(467), eaah5381.
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2

Autophagy Signaling Pathway Assay

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Antibodies to Akt, phospho-Akt (Ser473), phospho-Akt (Thr308), beclin-1, phospho-Beclin-1 (Ser15), phospho-PI3Kinase p85(Tyr458)/p55 (Tyr199), phospho-4E-BP1 (Thr37/46), p70S6K, phospho-p70S6K(Thr389), LC3B, poly(ADP-ribose) polymerase (PARP), cleaved PARP (D214) and anti-rabbit IgG HRP-linked antibody were purchased from Cell Signaling Technology (Beverly, MA, USA). Mouse monoclonal β-Actin antibody and crude fucoidan from F. vesiculosus were obtained from Sigma-Aldrich (St Louis, MO, USA). DMEM (Dulbecco’s Modification of Eagle’s Medium) with 4.5 g/L glucose, L-glutamine and sodium pyruvate, trypsin-EDTA, penicillin-streptomycin-amphotericin B solution (50X), fetal bovine serum (FBS), phosphate buffer solution (PBS), and PBS with Tween 20 (PBST) were purchased from Life Technologies (Waltham, MA, USA).
Zhang J., Riby J.E., Conde L., Grizzle W.E., Cui X, & Skibola C.F. (2016). A Fucus vesiculosus extract inhibits estrogen receptor activation and induces cell death in female cancer cell lines. BMC Complementary and Alternative Medicine, 16, 151.
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3

Culturing U87MG Glioblastoma Cells

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Studies were carried out in the human glioblastoma cell line U87MG (HTB-14). They were cultured in Dulbecco's Modified Eagle Medium (DMEM) with stable L-glutamine and sodium pyruvate (Life Technologies, Cat No:22320-030) supplemented with 10% fetal bovine serum (Life Technologies, Cat No:10082-147) without antibiotics in a humidified incubator at 37°C and 5% CO 2 atmosphere. Confluent cells were detached with trypsin-ethylenediaminetetraacetic acid (EDTA) solution (Life Technologies, Cat No:R-001-100) in calcium-free phosphate buffered saline (PBS) and counted in a Neubauer chamber.
Gokturk D., Kelebek H., Ceylan S, & Yilmaz D.M. (2018). The Effect of Ascorbic Acid over the Etoposide- and Temozolomide-Mediated Cytotoxicity in Glioblastoma Cell Culture: A Molecular Study. Turkish neurosurgery, 28(1).
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4

Culturing C2C12 and 3T3 Cells

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C2C12 derivative lines were cultured in high glucose Dulbecco’s Modified Eagle Media (DMEM) supplemented with 20% fetal bovine serum (FBS, Atlanta Biologicals), L-glutamine and sodium pyruvate (Gibco), penicillin and streptomycin (P/S, Gibco) at 37 °C in 5% O2/5% CO2. 3T3 derivatives were cultured in DMEM supplemented with 10% FBS and P/S.
Choi S.H., Bosnakovski D., Strasser J.M., Toso E.A., Walters M.A, & Kyba M. (2016). Transcriptional inhibitors identified in a 160,000-compound small molecule DUX4-viability screen. Journal of biomolecular screening, 21(7), 680-688.
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5

Culturing HEK293, HEK293S, and Sf9 Cells

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Adherent HEK293T cells (CRL-3216, American Tissue Culture Collection) were cultured in 10-cm Petri dishes in Dulbecco’s modified Eagle medium with l-glutamine and sodium pyruvate (Gibco), supplemented with 10% fetal bovine serum (FBS) and antibiotic–antimycotic at 37 °C and 5% CO2. Suspension HEK293S GnTI− cells (CRL-3022, American Tissue Culture Collection) were maintained in Freestyle medium with GlutaMAX (Gibco) supplemented with 1% FBS and antibiotic–antimycotic solution, at 37 °C, 5% CO2 and 60% humidity, in TPP600 bioreactors. Sf9 cells (12659017, ThermoFisher Scientific) were cultured in SFMIII medium supplemented with antibiotic–antimycotic, at 27 °C.
Kalienkova V., Dandamudi M., Paulino C, & Lynagh T. (2024). Structural basis for excitatory neuropeptide signaling. Nature Structural & Molecular Biology, 31(4), 717-726.
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6

Cell Culture of C2C12 and Variants

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C2C12 cells and the inducible C2C12 cell lines (iC2C12-DUX4 and iC2C12-Luc) were cultured in proliferation medium comprising high glucose Dulbecco’s Modified Eagle Media (DMEM) with L-glutamine and sodium pyruvate (Gibco), penicillin and streptomycin (P/S, Gibco), and 20% fetal bovine serum (FBS, Atlanta Biologicals) at 37°C in 5% O2/5% CO2. The i3T3-DUX4 cell line was cultured in DMEM, P/S, and 10% FBS.
Bosnakovski D., Choi S.H., Strasser J.M., Toso E.A., Walters M.A, & Kyba M. (2014). High-throughput screening identifies inhibitors of DUX4-induced myoblast toxicity. Skeletal Muscle, 4, 4.
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7

HEK293 Cell Culture Protocol

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HEK293 cells (ATCC, CRL-1573) were maintained in DMEM-C, high-glucose DMEM supplemented with L-glutamine and sodium pyruvate (Gibco 1195065), 10% FBS (Sigma F0926) and 1% pen-strep (Gibco 15140122). HEK293FT cells (Invitrogen R70007) for lentiviral packaging were maintained in DMEM-C containing 500 μg/ml geneticin (Gibco 10131035). Cells were grown at 5% CO2 at 37 °C.
Rudd J.C., Maity S., Grunkemeyer J.A., Snyder J.C., Lovas S, & Hansen L.A. (2023). Membrane structure and internalization dynamics of human Flower isoforms hFWE3 and hFWE4 indicate a conserved endocytic role for hFWE4. The Journal of Biological Chemistry, 299(8), 104945.
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