The bacterial strains and plasmids used in this study are described in Supplementary Table S31 . Genomic DNA from Helicobacter pylori HPKTCC B0100 was obtained from the Korean Type Culture Collection (KTCC), Korea National Research Resource Bank (KNRRC). The genomic DNA from Campylobacter jejuni NCTC11168 was kindly provided by Dr. Jong-Hyun Kim at the Centers for Disease Control and Prevention. E. coli strains/mutants DH5α, EPI-300, JP1111 (obtained from E. coli genetic resources at Yale; CGSC) and CL37 (kindly provided by Dr. John Cronan at the University of Illinois at Urbana-Champaign) were routinely grown at 37 °C in Luria-Bertani (LB) broth or LB agar supplemented with appropriate antibiotics. The antibiotic concentrations used were as follows; ampicillin, 100 μg/ml; kanamycin, 50 μg/ml; chloramphenicol, 30 μg/ml and TCS, 0.1–650 μg/ml. Fosmid pCC1FOS (Epicentre Biotechnologies, Madison, WI, USA) was used to construct genomic libraries, whereas pUC119 and pGEM®-T Easy were used for further subcloning experiments.
Fosmid pcc1fos
Manufactured by Illumina
Sourced in United States
The Fosmid pCC1FOS is a cloning vector used for the construction of genomic libraries. It is a bacterial artificial chromosome (BAC) vector derived from the F-factor of Escherichia coli, capable of maintaining large DNA inserts of up to 40 kilobases in size. The Fosmid provides a stable and controlled system for propagating and maintaining these large DNA fragments within the host cell.
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Lab products found in correlation
2 protocols using fosmid pcc1fos
1
Bacterial Genomic DNA Isolation and Plasmid Construction
2
Triclosan Resistance in Aeromonas salmonicida
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The bacterial strains and plasmids used in this study are described in Table 1. E. coli strains DH5α and EPI-300 were routinely grown at 37°C in Luria-Bertani (LB) broth or LB agar, while A. salmonicida subsp. salmonicida strain 14791 (AS 14791) was grown at 25°C on tryptone soya medium (Oxoid, UK) supplemented with the desired antibiotics. The antibiotic concentration used for susceptibility testing was as follows: ampicillin 100 µg/ml; tetracycline 20 µg/ml; chloramphenicol 30 µg/ml; and Triclosan 1-100 µg/ml. Triclosan was purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Fosmid pCC1FOS (Epicentre, USA) was used to construct the genomic library, whereas pUC119 and pRK415 were used for further subcloning experiments. The AS 14791 strain was obtained from the Korean Agricultural Culture Collection. In order to examine its resistance to Triclosan, this strain was grown on tryptone soya medium supplemented with 20 µg/ml of Triclosan.
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