Tmb 3 3 5 5 tetramethylbenzidine substrate
TMB (3,3′,5,5′-tetramethylbenzidine) is a chromogenic substrate used in enzyme-linked immunosorbent assays (ELISA) and other colorimetric detection methods. It serves as a substrate for horseradish peroxidase (HRP), an enzyme commonly used as a label in immunoassays. When TMB is oxidized by HRP in the presence of hydrogen peroxide, it produces a blue-colored product that can be measured spectrophotometrically.
2 protocols using tmb 3 3 5 5 tetramethylbenzidine substrate
CHIKV E2 and Virus Binding Assay
Serological Assay for Hantavirus Antibodies
The Gn and Gc proteins of HTNV and SEOV were coated similarly to identify different types of immunoglobulins. Mouse serum samples were diluted, and HRP-labeled IgG was used as IgG1, IgG2a, IgG2b, IgA and IgM (Southern Biotech, USA). The absorbance at 450 nm was then measured to detect the quantity of each immunoglobulin isotype.
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