Tubulin 9f3

Manufactured by Cell Signaling Technology

Sourced in United States

Tubulin (9F3) is a monoclonal antibody produced by Cell Signaling Technology. It recognizes the tubulin protein, a key structural component of the cytoskeleton in eukaryotic cells.

Automatically generated - may contain errors

Lab products found in correlation

Psma 1h8h5, by Thermo Fisher Scientific (2 mentions) Gapdh gt239, by GeneTex (2 mentions) Mglur1, by Merck Group (2 mentions) Calnexin c5c9, by Cell Signaling Technology (2 mentions) Cellytic m lysis buffer, by Merck Group (2 mentions) Pierce ecl western blotting substrate, by Thermo Fisher Scientific (2 mentions) Pierce bca protein assay, by Thermo Fisher Scientific (2 mentions) Phosstop, by Roche (2 mentions) Sc 32879, by Santa Cruz Biotechnology (1 mentions) Pvdf membrane, by Merck Group (1 mentions)

Spelling variants of this product

β-tubulin (9F3, (15 citations) Beta-Tubulin (9F3) (3 citations) Anti-β-Tubulin (9F3, (3 citations) Rabbit anti-β-tubulin 9F3 (2 citations) β-tubulin-AF647 (9F3, (2 citations)

3 protocols using tubulin 9f3

Western Blot Protein Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were washed with ice-cold PBS, harvested by scraping and then homogenized in CelLytic M lysis buffer (Sigma, Merck) supplemented with phosphatase and protease inhibitors (PhosSTOP and cOmplete, Roche). Lysates were incubated (1 hour, 4°C) and then centrifuged at 14,000 × g (10 minutes, 4°C). Protein samples were quantified using the Pierce BCA Protein Assay (Thermo Fisher Scientific), resolved by SDS-PAGE and transferred to polyvinylidene difluoride membranes (Millipore, Merck). Membranes were blocked with Tris-buffered saline/0.5% Tween 20 (TBST, pH 7.5) containing 5% skim milk powder and probed overnight with primary antibodies against the following targets: PSMA (1H8H5, Thermo Fisher Scientific), EGFR (D38B1), HER2 (44E7), HER3 (D22C5), p-AKT(S473) (587F11), p-AKT (T308) (244F9), AKT (D38B1), p-S6 (S235/236) (D57.2.2E), Calnexin (C5C9) and Tubulin (9F3) were from Cell Signaling Technology, AR (Santa Cruz Biotechnology), GAPDH (GT239, GeneTex), and mGluR1 (07-617, Merck). Following incubation with horseradish peroxidase–linked secondary antibodies (Dako, Agilent Technologies), immunoreactive bands were visualized with Pierce ECL Western Blotting Substrate (Thermo Fisher Scientific).

Gómez V., Galazi M., Weitsman G., Monypenny J., Al-Salemee F., Barber P.R., Ng K., Beatson R., Szokol B., Orfi L., Mullen G., Vanhaesebroeck B., Chowdhury S., Leung H.Y, & Ng T. (2022). HER2 Mediates PSMA/mGluR1-Driven Resistance to the DS-7423 Dual PI3K/mTOR Inhibitor in PTEN Wild-type Prostate Cancer Models. Molecular Cancer Therapeutics, 21(4), 667-676.

+ Open protocol

+ Expand

Quantitative Protein Analysis in Frozen Liver

Check if the same lab product or an alternative is used in the 5 most similar protocols

Frozen liver tissues were homogenized and immunoblotting was performed using rabbit anti-G6PC (custom made) dilution 1:5000 and rabbit anti-PEPCK (H-300) dilution 1:7000 (sc-32879) (Santa Cruz Biotechnology, Dallas, TX, USA) and normalized to the housekeeping gene tubulin (9F3) dilution 1:1000 (2128) (Cell Signaling, Danvers, MA, USA). The primary antibodies were detected with corresponding secondary HRP-conjugated goat anti-rabbit IgG (170-5046) (Biorad, Hercules, CA, USA) antibodies (dilution 1:10,000). The signal was quantified using the software ImageJ.

Lee Y.S., De Vadder F., Tremaroli V., Wichmann A., Mithieux G, & Bäckhed F. (2016). Insulin-like peptide 5 is a microbially regulated peptide that promotes hepatic glucose production. Molecular Metabolism, 5(4), 263-270.

+ Open protocol

+ Expand

Comprehensive Protein Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were washed with ice-cold PBS, harvested by scraping and then homogenized in CelLytic M lysis buffer (Sigma, Merck) supplemented with phosphatase and protease inhibitors (PhosSTOP and cOmplete, Roche, Basel, Switzerland). Lysates were incubated (1 h, 4°C) and then centrifuged at 14,000 x g (10 min, 4°C). Protein samples were quantified using the Pierce BCA Protein Assay (ThermoFisher Scientific), resolved by SDS-PAGE and transferred to PVDF membranes (Millipore, Merck). Membranes were blocked with Tris-buffered saline/0.5% Tween 20 (TBST, pH 7.5) containing 5% skim milk powder and probed overnight with primary antibodies against the following targets: PSMA (1H8H5, ThermoFisher Scientific), EGFR (D38B1), HER2 (44E7), HER3 (D22C5), p-AKT(S473) (587F11), p-AKT (T308) (244F9), AKT (D38B1), p-S6 (S235/236) (D57.2.2E), Calnexin (C5C9) and Tubulin (9F3) were from Cell Signaling Technology, MA, USA, AR (Santa Cruz Biotechnology, TX, USA), GAPDH (GT239, GeneTex, CA, USA) and mGluR1 (07-617, Merck). Following incubation with horseradish peroxidase-linked secondary antibodies (Dako, Agilent Technologies, CA, USA), immunoreactive bands were visualized with Pierce ECL Western Blotting Substrate (ThermoFisher Scientific).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!