Nebnext hifi 2x pcr master mix

Manufactured by New England Biolabs

Sourced in Switzerland

NEBNext HiFi 2x PCR Master mix is a pre-mixed solution designed for high-fidelity PCR amplification. It contains a high-performance DNA polymerase, optimized buffer, and necessary reagents for efficient and accurate DNA amplification.

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Lab products found in correlation

Kapa library quantification kit, by Roche (1 mentions) High seq platform, by Illumina (1 mentions) Novaseq 6000 platform, by Illumina (1 mentions) Minelute cleanup kit, by Qiagen (1 mentions) Tagment dna enzyme, by Illumina (1 mentions)

Spelling variants of this product

PCR master mix (27 citations) NEBnext PCR master mix (12 citations) NEBnext 2x master mix (8 citations) HiFi 2x PCR Master mix (2 citations) 2× HiFi PCR master mix (2 citations)

2 protocols using nebnext hifi 2x pcr master mix

Barcoded Cut&Tag Library Preparation

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Barcoded Cut&Tag libraries were prepared using NEBNext HiFi 2x PCR Master mix (NEB) with Nextera i7 and i5 dual index primers, purified with Mag-Bind® TotalPure NGS beads, quantified via qPCR using the KAPA Library Quantification Kit (Roche, Basel, Switzerland). Pooled libraries were sequenced on the Illumina High-seq platform (Read 1:150 cycles, Index 1:8 cycles, Index 2:8 cycles, Read 2:150 cycles).

Murakami S., White S.M., McIntosh A.T., Nguyen C.D, & Yi C. (2023). Spontaneously evolved progenitor niches escape Yap oncogene addiction in advanced pancreatic ductal adenocarcinomas. Nature Communications, 14, 1443.

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ATAC-seq profiling of Drosophila S2 cells

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2 × 10⁵Drosophila S2 cells were washed once with 1X PBS and then resuspended in 100 μL ATAC lysis buffer (10mM Tris 7.5, 10mM NaCl, 3mM MgCl2, 0.1% NP-40). Cells were centrifuged at 600 × g for 10 min at 4°C. The resulting pellet was resuspended in 47.5 μL buffer TD (Illumina 15027866) before adding 2.5 μL Tn5 transposase (Tagment DNA Enzyme, Illumina 15027865) and incubating in 37°C water bath for 30 min. The tagmented DNA was immediately purified using MinElute Cleanup Kit (QIAGEN 28204) and eluted in 10 μL buffer EB. Tagmented DNA was amplified with 12 cycles of PCR using the NEBNext Hi-Fi 2X PCR Master Mix (NEB M0541) and unique dual index primers. Libraries were purified using a 1.2X ratio of Axygen magnetic beads. 150bp, paire-end sequencing was performed at the University of Wisconsin-Madison Biotechnology Center on the Illumina Nova Seq 6000 platform.

Jain S.U., Rashoff A.Q., Krabbenhoft S.D., Hoelper D., Do T.J., Gibson T.J., Lundgren S.M., Bondra E.R., Deshmukh S., Harutyunyan A.S., Juretic N., Jabado N., Harrison M.M, & Lewis P.W. (2020). H3 K27M and EZHIP Impede H3K27-Methylation Spreading by Inhibiting Allosterically Stimulated PRC2. Molecular cell, 80(4), 726-735.e7.

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