6110 quadrupole

LC-MS analysis was conducted on lipid nanodiscs using Agilent Technologies 1200 Series Instrument with a G1316A variable wavelength detector set at λ = 210 nm, 1200 Series ELSD, 6110 quadrupole ESI-MS, using an Agilent Zorbax Eclipse XDB-Phenyl column (3 × 100 mm, 3.5 μm particle size, flow rate 1 mL/min, the mobile phases 0.05% formic acid in water and 0.05% formic acid in acetonitrile).

Rhamnolipid mixtures were separated and identified by liquid chromatography coupled to mass spectroscopy using an Agilent Technologies 6110 quadrupole LC–MS (Smyth et al., 2010 (link)). Samples were prepared with ACN:H₂O (80:20 v/v; LC grade) with a concentration of 10 mg/l and 100 μl of the same was injected into a C₁₈ (150mm × 2.1mm × 5 μm) column. The LC flow rate was 0.25 ml/min. For mobile phase, an acetonitrile–water gradient was used starting with 40% of acetonitrile for 4 min, followed by 40–90% acetonitrile in 20 min then return to the initial condition in 6 min. Ammonium acetate buffer was added.