The following reagents were used: EMDM (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA); FBS (Gemini Bio Products, West Sacramento, CA, USA); TRIzol (Ambion; Thermo Fisher Scientific, Inc.); MDC (Tiangen Biotech Co., Ltd., Beijing, China); WIP organization cell lysis solution (Boaoseng, Beijing, China); RNase Away (Ed lai, Beijing, China); BCA protein concentration determination kit (Blue skies, China); SDS-PA (Nanjing KeyGen Biotech. Co. Ltd., Nanjing, China); Reverse transcription kit (Lisu, Shanghai, China); Primers (Hongxun, Suzhou, China); High sensitive chemiluminescence detection kit (Qihaifutan, Shanghai, China); TRAP1 Rabbit mAb (Cell Signaling Technology, Inc., Danvers, MA, USA); Beclin1 Rabbit mAb (Cell Signaling Technology, Inc.); LC3 Rabbit mAb (Cell Signaling Technology, Inc.); Anti-rabbit IgG, HRP-linked Antibody (Isotype: Goat; Cell Signaling Technology, Inc.); Mouse anti β-actin mAb (Zhongshanjinqiao, China); Anti-β-actin Mouse Monoclonal Antibody (Isotype: Goat; Zhongshanjinqiao, China); and Protein Simple (San Jose, CA, USA).
Beclin1 rabbit mab
Manufactured by Cell Signaling Technology
Sourced in United States
Beclin1 Rabbit mAb is a monoclonal antibody that recognizes the Beclin1 protein. Beclin1 is a key regulator of autophagy, a cellular process that involves the degradation and recycling of damaged or unnecessary cellular components.
Automatically generated - may contain errors
Lab products found in correlation
Lc3 rabbit mab, by Cell Signaling Technology (1 mentions)
Mouse anti β actin mab, by Cell Signaling Technology (1 mentions)
Anti β actin mouse monoclonal antibody, by Cell Signaling Technology (1 mentions)
Anti rabbit igg hrp linked antibody, by Cell Signaling Technology (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Gemini Bio (1 mentions)
Caspase 9 antibody, by Cell Signaling Technology (1 mentions)
Mouse igg2a isotype control, by Abcam (1 mentions)
P21 waf1 cip1 12d1 rabbit mab, by Cell Signaling Technology (1 mentions)
Rapamycin, by Merck Group (1 mentions)
Chloroquine, by Merck Group (1 mentions)
Bapta am, by Thermo Fisher Scientific (1 mentions)
Propofol, by Merck Group (1 mentions)
Bafilomycin, by Merck Group (1 mentions)
Mtt 3 4, by Merck Group (1 mentions)
Rpmi 1640 culture media, by Thermo Fisher Scientific (1 mentions)
Gapdh antibody, by Abcam (1 mentions)
Fura 2 am, by Thermo Fisher Scientific (1 mentions)
Dmem f12, by Thermo Fisher Scientific (1 mentions)
Retinoic acid, by Merck Group (1 mentions)
3 protocols using beclin1 rabbit mab
1
Autophagy Pathway Regulation in Cell Lines
2
Comprehensive Cellular Assay Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The source of materials adopted in this article
can be found in our previous reported article.11 (link) In addition, the flow cytometry (FCM) anti-P-glycoprotein
antibody and mouse IgG2a isotype control were supplied by Abcam (Cambridge,
UK). The anti-P-glycoprotein antibody, anti-cyclin A2 antibody, anti-β
actin antibody, and anti-β tubulin antibody were offered by
Abcam. The anti-cyclin D1 antibody was supplied by Beyotime (Shanghai,
China). P21 Waf1/Cip1 (12D1) rabbit mAb, the caspase-9 antibody, the
caspase-3 antibody, LC3A/B rabbit mAb, the Beclin-1 rabbit mAb, and
Atg12 rabbit mAb were obtained from the Cell Signaling Technology,
Inc. (Shanghai, China). All other chemicals unless otherwise indicated
were of analytical grade from Alladin Co. Ltd (Shanghai, China).
can be found in our previous reported article.11 (link) In addition, the flow cytometry (FCM) anti-P-glycoprotein
antibody and mouse IgG2a isotype control were supplied by Abcam (Cambridge,
UK). The anti-P-glycoprotein antibody, anti-cyclin A2 antibody, anti-β
actin antibody, and anti-β tubulin antibody were offered by
Abcam. The anti-cyclin D1 antibody was supplied by Beyotime (Shanghai,
China). P21 Waf1/Cip1 (12D1) rabbit mAb, the caspase-9 antibody, the
caspase-3 antibody, LC3A/B rabbit mAb, the Beclin-1 rabbit mAb, and
Atg12 rabbit mAb were obtained from the Cell Signaling Technology,
Inc. (Shanghai, China). All other chemicals unless otherwise indicated
were of analytical grade from Alladin Co. Ltd (Shanghai, China).
3
Autophagy Induction and Inhibition Assay
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+ Expand
DMEM/F-12, RPMI1640 culture media, BAPTA-AM, Fura2-AM and B27 were obtained from Life Technologies (New York city, NY, USA). MTT (3-(4, 5-dimethyithiazol-2-yl)-2,5-diphenyl-tetrazolium bromide), propofol, rapamycin, bafilomycin, retinoic acid, 3-MA, chloroquine and TMRE were purchased from Sigma (Saint Louis, MO, USA). 30% Acrylamide solution and nitrocellulose membrane (0.45µm) were obtained from Bio-Rad (Hercules, CA, USA). LC3B rabbit polyclonal Ab (#3868), beclin-1 rabbit mAb (#3495), AMPK rabbit mAb (#2603), p-AMPK rabbit mAb (#2535), p-S6K rabbit polyclonal Ab (#9205), LAMP1 (D2D11) XP Rabbit ab (#9091), and cleaved caspase-3 rabbit polyclonal ab (#9661) were obtained from Cell Signaling (Danvers, MA, USA) and used in 1:1000 dilution. p62 mouse monoclonal antibody (#28359,1:1000) was purchased from Santa Cruz (Dallas, TX, USA). GAPDH antibody (#9484, 1:1000) was acquired from Abcam (Cambridge, MA, USA).
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