Fts 6000 spectrometer vertex 70 70v

Structural studies of the obtained biomaterials were performed using Fourier transform infrared (FTIR) spectroscopy, within the scanning range of 400–4000 cm⁻¹ and resolution of 4 cm⁻¹ using a BioRad FTS 6000 spectrometer (Vertex 70&70v, Bruker, Billerica, MA, USA). Positions of the FTIR bands were measured in accordance with the center of weight. The baseline correction, normalization, and spectral analyses were performed using Spectragryph software (v1.2.15, Friedrich Menges, Oberstdorf, Germany). The measurements were carried out after 7 days of setting and hardening in air, as well as after 7 days of incubation in simulated body fluid (SBF).

The crystalline phases were analysed by a powder X-ray diffractometer (XRD, D2 Phaser, Bruker, Ballerica, MA, USA). An XRD analysis was performed using CuK-α radiation (1.54 Å) at 30 kV and 10 mA. The intensity was recorded in a 2θ range from 10° to 90° at 0.04° intervals with a scanning speed of 2.5° min⁻¹. The crystalline phases were identified by comparing the experimental diffractograms to the Joint Committee on Powder Diffraction Standards: α-TCP (JCPDS 00-009-0348) and hydroxyapatite (HAp; JCPDS 01-076-0694). A quantitative phase composition analysis based on Rietveld refinement was performed using Profex software (Version 4.3.5., Nicola Döbelin, Solothurn, Switzerland). The identification and quantification of the crystalline structures of materials nonincubated and incubated in simulated body fluid (SBF) at 37 °C were made after 7 days of setting and hardening.
Structural analyses were performed by Fourier transform infraRed spectroscopy (FTIR), within the scanning range 400–4000 cm⁻¹ and resolution of 4 cm⁻¹ using a BioRad FTS 6000 spectrometer (Vertex 70&70v, Bruker, Ballerica, MA, USA). The band positions of each result were measured according to the center of weight. The baseline correction, normalisation, and spectra analyses were performed using the Spectragryph software (Vwrsion v1.2.15, Friedrich Menges, Oberstdorf, Germany).