Soil pH was measured in a dry soil: H2O ratio of 1:2.5 (w/v) with a XL60 pH meter (Fisher Scientific, Asheville, USA)28 (link). The water content (%) of soil was determined gravimetrically by drying the fresh soils at 105 °C for 16 h. A laser particle-size analyzer (Mastersizer 2000, Malvern Instruments Ltd., Malvern, UK) was used to measure the contents of clay, loam and sand. The soils were sieved through a 0.15-mm mesh, and total C, N and sulfur (S) contents were measured with an elemental analyzer (vario MAX CN, Elementar, Hanau, Germany). NH4+ and NOx− were extracted with 2 M KCl at a soil:KCl ratio of 1:10 and filtered through a 0.22-μm hydrophilic PEPT needle filter (ANPEL, Shanghai, China). The filtrates were analyzed by a flow injection analyzer (QuikChem 8500, Lachat Instruments, USA). Five grams of soil were digested in sulfuric acid29 (link) for determining total potassium (K), calcium (Ca), sodium (Na), magnesium (Mg), iron (Fe) and aluminum (Al) contents by inductively coupled plasma optical emission spectrometry (ICP-OES) (Optima 7000DV, Perkin Elmer, Waltham, USA). The contents of total P and available P (AP) were determined using the molybdate-blue method30 (link) and sodium bicarbonate extraction31 , respectively.
Xl60 ph meter
Manufactured by Thermo Fisher Scientific
Sourced in United States
The XL60 pH meter is a laboratory instrument designed to measure the pH of a solution. It provides accurate and reliable pH measurements, enabling users to monitor and control the acidity or alkalinity of their samples.
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Lab products found in correlation
3 protocols using xl60 ph meter
1
Soil Characterization Techniques
2
Measuring iPSB Screening Indicators
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The pH and soluble-P concentration of the medium after incubation were used as indices for iPSB screening. The indices were measured by incubating all strains in 50 mL of liquid modified PVK (without agar and indicator) at 30 °C for 72 h. The supernatants obtained after centrifugation (4200g for 10 min) were used to measure pH with a XL60 pH meter (Fisher Scientific, USA) and phosphate concentration using the molybdate-blue method (Murphy and Riley 1962 (link)).
3
Characterization of Changshu Agricultural Soil
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Soil samples (0-15 cm layer) were collected in May 2015 after crop harvest from the Changshu AgroEcological Experimental Station in Jiangsu Province, China (31°32'N, 120°41'E). The soil contained 0.78% clay, 18.38% silt and 80.84% sand.
The samples were air-dried, sieved (0.2 mm) and stored at 4 °C and -20 °C until use and analysis.
Soil pH was determined in a suspension (1:2.5 dry soil:sterilized water (w/v)) using a XL60 pH meter (Fisher Scientific, Asheville, USA). Soil moisture content (%) was calculated as the difference in weight between fresh soil and soil dried in an oven at 300 °C for 24 h. Carbon, nitrogen and sulfur contents were measured with an elemental analyzer (vario MAX CNS, Elementar, Hanau, Germany). P and available-P contents were determined after digestion by strong acid (sulfuric acid) and extraction by sodium bicarbonate using the molybdate-blue method (Murphy and Riley, 1962; Olsen et al., 1954; Parkinson and Allen, 1975) , respectively. The acid-digested soils were further used to determine the concentrations of metals (potassium, calcium, sodium, magnesium, iron and aluminum) by inductively coupled plasma optical emission spectrometry (ICP-OES, Optima 7000DV, Perkin Elmer, Waltham, USA).
The physical and chemical properties of the Changshu soil are listed in Table S1.
The samples were air-dried, sieved (0.2 mm) and stored at 4 °C and -20 °C until use and analysis.
Soil pH was determined in a suspension (1:2.5 dry soil:sterilized water (w/v)) using a XL60 pH meter (Fisher Scientific, Asheville, USA). Soil moisture content (%) was calculated as the difference in weight between fresh soil and soil dried in an oven at 300 °C for 24 h. Carbon, nitrogen and sulfur contents were measured with an elemental analyzer (vario MAX CNS, Elementar, Hanau, Germany). P and available-P contents were determined after digestion by strong acid (sulfuric acid) and extraction by sodium bicarbonate using the molybdate-blue method (Murphy and Riley, 1962; Olsen et al., 1954; Parkinson and Allen, 1975) , respectively. The acid-digested soils were further used to determine the concentrations of metals (potassium, calcium, sodium, magnesium, iron and aluminum) by inductively coupled plasma optical emission spectrometry (ICP-OES, Optima 7000DV, Perkin Elmer, Waltham, USA).
The physical and chemical properties of the Changshu soil are listed in Table S1.
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