7890b gc system 5977a msd

Gas chromatography-mass spectrometry (GC/MS) with a 7890B GC System/5977A MSD (Agilent Technologies, Inc., Santa Clara, CA, USA) and 5 μm filters (Millipore, County Cork, Ireland) was used to determine the presence of IA, IAA, or CA in lipid vesicles. The chemical 3-octanol was added as the internal standard to the lipid vesicle solution with 50% of IA, IAA, or CA, and sodium chloride was measured using stir-bar sorptive extraction [5 (link)]. To prevent flavors presence out of the membranes, the filter was caught because it is not possible to simply attach a filter even if the flavor compounds are present in the solution before they reach the membranes. As a comparison, we made a solution by mixing flavors and DOPC. When preparing these solutions, sodium chloride was added to promote extraction [5 (link)]. Each experiment was conducted three times.

Headspace samples were analyzed by GC-MS (7890B GC System, 5977A MSD, Agilent Technologies, https://www.agilent.com) equipped with a polar column (HP-INNOWAX, 30 m long, 0.25 mm inner diameter, 25 µm film thickness; Agilent) with helium as carrier gas. The inlet temperature was set to 240°C. The temperature of the GC oven was held at 40°C for 3 min, and then increased by 10°C per min to 260°C. This final temperature was held for 15 min. The MS transferline was held at 260°C, the MS source at 230°C, and the MS quad at 150°C. Mass spectra were taken in electron ionization mode (70 eV) in the range from m/z 29 to 350. GC-MS data were processed with the MDS-ChemStation Enhanced Data Analysis software (Agilent).

Gas chromatography–mass spectrometry (GC/MS) was performed using a 7890B GC System/5977A MSD (Agilent Technologies, Inc., United States) and 10 μm filters (Toyo Roshi Kaisha Ltd., Japan) to determine the presence of EC in lipid vesicles. Methyl caproate was added as internal standard to the 50% EC lipid vesicle solution (solution A in Figure 1), and sodium chloride was measured using stir-bar sorptive extraction [22 (link)]. To prevent EC presence out of the membranes, the filter was caught because it is not possible to simply attach a filter even if the EC is present in the solution before it reaches the membranes. As a comparison, we made a solution by mixing EC and DOPC (solution C in Figure 1). When preparing these solutions, sodium chloride was added to promote extraction [22 (link)]. Each experiment was conducted three times.