Quant it picogreen assay system

Nextera XT libraries were prepared manually following the manufacturer’s protocol (Illumina). Briefly, samples were normalized to 0.2 ng/μl DNA material per library using a Quant-iT picogreen assay system (Life Technologies) on an AF2200 plate reader (Eppendorf), then fragmented and tagged via tagmentation. Amplification was performed by Veriti 96 well PCR (Applied Biosystems) followed by AMPure XP bead cleanup (Beckman Coulter). Fragment size was measured using Labchip GX Touch high-sensitivity. For cluster generation and next generation sequencing, samples were normalized to 1 nM, pooled, and diluted to 8 pM. The paired-end cluster kit V4 was used and cluster generation was performed on an Illumina cBot, with pooled samples in all 8 lanes. Sequencing was performed on an Illumina HiSeq. 2500 using SBS kit V4 chemistry. Median cluster densities (K mm2) were 908.5 for Nextera XT.

Libraries were prepared manually following the manufacturer’s protocol (Illumina, PN. 15031942). Briefly, samples were normalized to 0.2 ng/μl DNA material per library using a Quant-iT picogreen assay system (Life Technologies, PN. Q33120) on an AF2200 plate reader (Eppendorf), then fragmented and tagged via tagmentation. Amplification was performed by Veriti 96 well PCR (Applied Biosystems) followed by AMPure XP bead cleanup (Beckman Coulter, PN. A63880). Fragment size was measured using Labchip GX Touch high-sensitivity.