Rabbit anti relb

Manufactured by Santa Cruz Biotechnology

Rabbit anti-RelB is a polyclonal antibody produced in rabbits against the RelB protein. RelB is a member of the NF-kB family of transcription factors and plays a role in the regulation of gene expression. This antibody can be used for the detection and analysis of RelB in various experimental applications.

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Lab products found in correlation

Mouse anti β actin, by Merck Group (2 mentions) Rabbit anti histone h3, by Cell Signaling Technology (1 mentions) Fluorophore conjugated antibody, by Thermo Fisher Scientific (1 mentions) Rabbit anti rela, by Santa Cruz Biotechnology (1 mentions) Rabbit anti iκbα, by Cell Signaling Technology (1 mentions) Hrp conjugated streptavidin, by Jackson ImmunoResearch (1 mentions) Nitrocellulose membrane, by GE Healthcare (1 mentions) Rabbit anti p akt, by Cell Signaling Technology (1 mentions) Rabbit anti akt, by Thermo Fisher Scientific (1 mentions) Ecl western blotting substrate, by Thermo Fisher Scientific (1 mentions) Supersignal west dura extended duration substrate, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Anti-RelB Rabbit anti-TM

2 protocols using rabbit anti relb

Antibody Reagents for Protein Analysis

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The following antibodies were used in this study: rabbit anti-RelA, rabbit anti-RelB (Santa Cruz Biotechnology, Santa Cruz, CA), rabbit anti-Histone H3, rabbit anti-IκBα (Cell Signaling Technology, Danvers, MA), mouse anti-GSH (Virogen, Watertown, MA), mouse anti-IKKα (Upstate, Millipore, Billerica, MA) Streptavidin conjugated-HRP (Jackson, West Grove, PA), goat anti-Grx1 (American Diagnostica, Stamford, CT) and mouse anti-β-actin (Sigma-Aldrich, Saint Louis, MO). The secondary HRP conjugated anti-rabbit and anti-mouse antibodies were from Amersham (Piscataway, NJ), anti-goat was from Jackson Laboratories (West Grove, PA). All fluorophore-conjugated antibodies were from Invitrogen (Carlsbad, CA).

Nolin J.D., Tully J.E., Hoffman S.M., Guala A.S., van der Velden J.L., Poynter M.E., van der Vliet A., Anathy V, & Janssen-Heininger Y.M. (2014). The glutaredoxin/S-glutathionylation axis regulates interleukin 17A-induced pro-inflammatory responses in lung epithelial cells in association with S-glutathionylation of Nuclear Factor kappa B family proteins. Free radical biology & medicine, 0, 143-153.

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Western Blot Analysis of RELB, p100/p52, AKT

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Purified B cells were subjected to NP40-based lysis, separated by SDS-PAGE and blotted on nitrocellulose membranes (GE Healthcare). Samples were incubated with the following primary antibodies overnight at 4°C: rabbit anti-RELB (Santa Cruz, clone: C-19), rabbit anti-p100/p52 (Cell Signaling Technologies, CST), mouse anti-β-actin (Sigma, clone: AC-15), rabbit anti-pAKT (Cell Signalling, clone: D9E) and rabbit anti-AKT (CST) Horseradish peroxidase-conjugated secondary antibodies and ECL Western Blotting Substrate or SuperSignal West Dura Extended Duration Substrate (Thermo Scientific) were used for detection.

De Silva N.S., Silva K., Anderson M.M., Bhagat G, & Klein U. (2016). Impairment of mature B-cell maintenance upon combined deletion of the alternative NF-κB transcription factors RELB and NF-κB2 in B cells. Journal of immunology (Baltimore, Md. : 1950), 196(6), 2591-2601.

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