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Glutathione (gsh)

Manufactured by NJJCBIO
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Sourced in Japan

The GSH is a laboratory instrument used for the measurement of glutathione (GSH) levels in biological samples. It is a versatile and reliable tool for researchers and scientists working in the fields of biochemistry, cell biology, and oxidative stress analysis.

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Lab products found in correlation

Nfe2l2 16396 1 ap, by Proteintech (1 mentions) Mitotracker, by Beyotime (1 mentions) Lyso tracker red, by Solarbio (1 mentions) Crystal violet staining solution, by Solarbio (1 mentions) Lc3ii 14600 1 ap, by Proteintech (1 mentions)

2 protocols using glutathione (gsh)

1

Multimodal Assessment of Cellular Stress

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NFE2L2 (16396-1-AP) and LC3II (14600-1-AP) were purchased from proteintech. HO-1 (86806, CST), FTH1 (4393, CST), Gpx4 (PA5-102521, Invitrogen), NCOA4 (HC071-1F11, Sigma-Aldrich).
Esculetin, Cisplatin and Sorafenib (MCE, USA), T-AOC Assay, ROS Assay Kit, Crystal Violet Staining Solution, Lyso-Tracker Red (Solarbio, China), Mito-Tracker (Beyotime, China), DPPH free radical scavenging rate detection kit (Leagene, China), TranswellTM polycarbonate membrane cell culture inserts (Corning, Germany), Liperfluo, FerroOrange, Cell Counting Kit-8 (DOJINDO, Japan), GSH (reduced glutathione) determination kit (NJJCBIO).
Xiu Z., Li Y., Fang J., Han J., Li S., Li Y., Yang X., Song G., Li Y., Jin N., Zhu Y., Zhu G., Sun L, & Li X. (2023). Inhibitory Effects of Esculetin on Liver Cancer Through Triggering NCOA4 Pathway-Mediation Ferritinophagy in vivo and in vitro. Journal of Hepatocellular Carcinoma, 10, 611-629.
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2

Antioxidant Levels in Brain Tissue and Cells

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The brain tissue was homogenized in physiological saline solution, and then the supernatants were obtained after centrifugation. SH-SY5Y cells were digested by pancreatic enzymes after different treatments. After centrifugation, the precipitates were dissolved in PBS by ultrasonication. Afterward, GSH, SOD or MDA detection kits from njjcbio were used following the corresponding manufacturing instructions. The absorbance was measured at 405 nm, 450 nm, or 532 nm. All experiments were performed at least in triplicate.
Yu J., Zhang Y., Zhu Q., Ren Z., Wang M., Kong S., Lv H., Xu T., Xie Z., Meng H., Han J, & Che H. (2024). A mechanism linking ferroptosis and ferritinophagy in melatonin-related improvement of diabetic brain injury. iScience, 27(4), 109511.
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