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Shamrock sr 750

Manufactured by Oxford Instruments

The Shamrock SR-750 is a high-performance spectrograph designed for a variety of spectroscopic applications. It features a focal length of 750 mm and a range of available gratings to cover different wavelength regions. The Shamrock SR-750 provides high-resolution, accurate, and reproducible spectral data.

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2 protocols using shamrock sr 750

1

Spectral Measurements of Collimated Jet Beams

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In spectral measurements, at location C in Fig. 2a the scattered light from one section of the collimated double jet beams (or the sideways emission) is coupled into a Shamrock spectrometer (Shamrock SR-750, Andor) equipped with an ICCD detector (DH740i-18U-03, iStar, Andor). In this case, in addition to the imaging lens, a couple of steering mirrors (not shown in Fig. 2a) are employed for rotating the sampled source section by 90-degree so that the image becomes parallel to the entrance slit of the spectrometer and so improved light coupling efficiency is achieved. Spectral measurements at the other three locations labeled by A, B and D respectively are made with a miniature fiber-pigtailed spectrometer (Model S2000, Ocean Optics) of lower sensitivity but more convenience of operation. In measuring the spectrum of the collimated jet beams (not the scattered light) at location D, three optical wedges are utilized to reflect off the beam three times and thus significantly reduce the optical power entering the spectrometer. It should be noted that for all the spectral traces given in this paper the spectral response of the detection system has not been corrected, and thus the relative heights of the spectral peaks, especially those in Fig. 1d, trace D in Fig. 2b do not present the real signal strength.
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2

Optical Characterization of AuNP-DNA Origami

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UV–vis characterization was performed using the set up described in our previous work [27 ]. Briefly an inverted optical microscope (Axiovert 200, Zeiss) in transmitted light illumination (HAL 100 illuminator, Zeiss) was coupled with a microscope with 750 mm long spectrometer (Shamrock SR-750, Andor Technology plc.). The distinguishable agarose gel band representing AuNP-DNA origami hybrid structures were cut out after the electrophoretic procedure and were placed onto clean glass coverslip then mounted on XY sample stage.
The light transmitted through the sample was collected by 100× immersion objective (NA 1.45, α Plan-FLUAR, Zeiss), directed into a spectrometer, split by a diffractive grating of 600 lines per mm, and finally analysed using TE-cooled EMCCD (Newton DU971-UVB, Andor Technology plc.). The extinction spectra obtained from highly concentrated AuNP were normalized using the extinction spectra of the same gel acquired in a position without AuNP.
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