Structural characterization of asprepared QDs was carried out using a JEOL 2200-FX analytical high-resolution transmission electron microscope with a 200 kV accelerating voltage. Samples for TEM were prepared by spreading a drop (5–10 μL at a concentration of ∼1 μM) of the filtered QD dispersion onto an ultrathin carbon support film on a 300 mesh Au grid (Ted Pella, Inc.) and letting it dry. Individual particle sizes were measured using a Gatan Digital Micrograph (Pleasanton, CA, USA). Average sizes along with standard deviations were extracted from analysis of ∼100 QDs.
300 mesh au grid
Manufactured by Ted Pella
The 300 mesh Au grid is a laboratory equipment item used for sample preparation in various microscopy techniques. It is made of gold (Au) and has a mesh size of 300, which refers to the number of openings per inch. This grid serves as a support for thin samples, allowing them to be easily transferred and observed under a microscope.
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Lab products found in correlation
5 protocols using 300 mesh au grid
1
TEM Characterization of Quantum Dots
2
Structural Characterization of AuNPs
3
Structural Characterization of Nanoparticles
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Structural characterization
of as-prepared NPs was carried out using a JEOL JEM-2100 FE-TEM, field-emission
gun transmission electron microscope, providing high-spatial-resolution
atomic imaging and microstructure analysis of material samples. Samples
for TEM were prepared by spreading a drop (5–10 μL) of
the filtered NP dispersion (filtered using 0.25 μm Millipore
syringe filters) onto an ultrathin carbon/holey support film on a
300 mesh Au grid (Ted Pella, Inc.) and letting it dry. The concentration
of NPs in the DI water used was typically ∼1 μM. Individual
particle sizes were measured using a Gatan DigitalMicrograph (Pleasanton,
CA); average sizes along with standard deviations were extracted from
the analysis of ∼100 NPs.
of as-prepared NPs was carried out using a JEOL JEM-2100 FE-TEM, field-emission
gun transmission electron microscope, providing high-spatial-resolution
atomic imaging and microstructure analysis of material samples. Samples
for TEM were prepared by spreading a drop (5–10 μL) of
the filtered NP dispersion (filtered using 0.25 μm Millipore
syringe filters) onto an ultrathin carbon/holey support film on a
300 mesh Au grid (Ted Pella, Inc.) and letting it dry. The concentration
of NPs in the DI water used was typically ∼1 μM. Individual
particle sizes were measured using a Gatan DigitalMicrograph (Pleasanton,
CA); average sizes along with standard deviations were extracted from
the analysis of ∼100 NPs.
4
Characterization of Quantum Dots and Gold Nanoparticles
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Three different techniques
were used to characterize the QDs and AuNP used in this study: (1)
Electronic absorption and PL emission spectra were recorded using
a Shimadzu UV-1800 UV–vis spectrophotometer and a Horiba, Inc.
fluorometer (excitation at λ = 395 nm), respectively. (2) Dynamic
light scattering (DLS) was used to measure hydrodynamic size. The
samples were transferred into a square-shaped capillary, and measurements
were recorded on a ZetaSizer Ultra instrument equipped with a HeNe
laser source (λ = 633 nm) (Malvern Instruments Ltd., Worcestershire,
UK) and analyzed using Dispersion Technology Software (Malvern Instruments
Ltd.) as previously described.20 (link) (3) Structural
characterization and elemental analysis of the as-prepared NPs were
carried out using a JEOL 2200-FX analytical high-resolution transmission
electron microscope with a 200 kV accelerating voltage. TEM samples
were prepared by spreading a drop (5–10 μL) containing
the NPs onto an ultrathin carbon/holey support film on a 300 mesh
Au grid (Ted Pella, Inc.) and letting it dry. The concentration of
NPs used for TEM was 50–100 nM. Individual particle sizes were
measured using a Gatan digital micrograph (Pleasanton, CA, USA); average
sizes along with standard deviations were extracted from analysis
of at least 50–100 nanoparticles.
were used to characterize the QDs and AuNP used in this study: (1)
Electronic absorption and PL emission spectra were recorded using
a Shimadzu UV-1800 UV–vis spectrophotometer and a Horiba, Inc.
fluorometer (excitation at λ = 395 nm), respectively. (2) Dynamic
light scattering (DLS) was used to measure hydrodynamic size. The
samples were transferred into a square-shaped capillary, and measurements
were recorded on a ZetaSizer Ultra instrument equipped with a HeNe
laser source (λ = 633 nm) (Malvern Instruments Ltd., Worcestershire,
UK) and analyzed using Dispersion Technology Software (Malvern Instruments
Ltd.) as previously described.20 (link) (3) Structural
characterization and elemental analysis of the as-prepared NPs were
carried out using a JEOL 2200-FX analytical high-resolution transmission
electron microscope with a 200 kV accelerating voltage. TEM samples
were prepared by spreading a drop (5–10 μL) containing
the NPs onto an ultrathin carbon/holey support film on a 300 mesh
Au grid (Ted Pella, Inc.) and letting it dry. The concentration of
NPs used for TEM was 50–100 nM. Individual particle sizes were
measured using a Gatan digital micrograph (Pleasanton, CA, USA); average
sizes along with standard deviations were extracted from analysis
of at least 50–100 nanoparticles.
5
Visualizing Quantum Dot Assembly
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A 10 μL aliquot of PURExpress Solution A was mixed with 7.5 μL of Solution B, H2O, and assembled and 75 nM QD (625 nm emission maxima) or water (control) at 4°C for 30 min. From the assembled reaction a 5–10 μL volume of the sample was spread onto ultrathin carbon/holey support film on a 300 mesh Au grid (Ted Pella, Inc.) and left to dry at room temperature. The samples were characterized using a JEOL 2200-FX analytical high-resolution transmission electron microscope (TEM) with a 200 kV accelerating voltage [47 (link), 85 (link)].
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