Blood was collected into serum separator tubes with clot activator. Samples were centrifuged at 3000 g for 15 min to separate serum. All specimens were stored at −80°C until analysis. Serum total ghrelin levels were measured with the commercially available ELISA kit (RayBio Human/Mouse/Rat Ghrelin Enzyme Immunoassay, RayBiotech, Norcross, GA, USA). The sensitivity limit of total ghrelin was 0.1 ng/mL. Serum glucose levels were measured using the autoanalyzer system (Aeroset System Abbott, Abbott Laboratories, Diagnostic Division, IL, USA) and HbA1c levels were determined by high performance liquid chromatography (Trinity Biotech, Kansas City, USA). Serum insulin levels were measured by means of ADVIA Centaur Chemiluminescence Enzyme Immunoassay (Siemens Healthcare Diagnostics, NY, USA) (for SI unit conversions: mg/dL × 0.0555 mmol/L for glucose, μIU/mL × 6.945 = pmol/L for insulin, and pg/mL × 0.3 = pmol/L for ghrelin).
Raybio human mouse rat ghrelin enzyme immunoassay
Manufactured by RayBiotech
Sourced in United States
The RayBio Human/Mouse/Rat Ghrelin Enzyme Immunoassay is a quantitative sandwich enzyme immunoassay kit designed for the detection and measurement of ghrelin levels in human, mouse, and rat samples.
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Lab products found in correlation
2 protocols using raybio human mouse rat ghrelin enzyme immunoassay
1
Ghrelin, Glucose, and Insulin Biomarker Analysis
2
Serum Ghrelin Levels and Metabolic Markers
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Blood was collected into serum separator tubes with clot activator. Samples were centrifuged at 3000 g for 15 min to separate serum. All specimens were stored at −80°C until analysis. Serum total ghrelin levels were measured with the commercially available ELISA kit (RayBio Human/Mouse/Rat Ghrelin Enzyme Immunoassay, RayBiotech, Norcross, GA, USA). The sensitivity limit of total ghrelin was 0.1 ng/mL and intra- and inter-assay coefficients of variability of the assay were <10% and <15%, respectively. Baseline laboratory parameters, including serum glucose, total cholesterol, triglycerides (TG) and high-density lipoprotein (HDL) cholesterol, were measured using the auto analyzer system (Aeroset System Abbott, Abbott Laboratories) and HbA1c levels were determined by high-performance liquid chromatography (Trinity Biotech, Kansas City, MO, USA). Low-density lipoprotein (LDL) cholesterol was calculated by the Friedewald formula as total cholesterol– (HDL cholesterol + TG/5) in case of measured TG level below 400 mg/dL. (For SI unit conversions: pg/mL × 0.3 = pmol/L for ghrelin).
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