Recombinant sars cov 2 nucleocapsid protein

Manufactured by RayBiotech

Sourced in United States, Gabon

The Recombinant SARS-CoV-2 nucleocapsid protein is a laboratory product that contains the nucleocapsid protein of the SARS-CoV-2 virus. The nucleocapsid protein is a structural protein that is responsible for packaging the viral genome within the virus particle.

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Lab products found in correlation

Streptavidin immunocaps, by Thermo Fisher Scientific (1 mentions) Recombinant sars cov 2 s1 subunit protein, by RayBiotech (1 mentions) Alexa fluor 546 protein labeling kit, by Thermo Fisher Scientific (1 mentions)

3 protocols using recombinant sars cov 2 nucleocapsid protein

SARS-CoV-2 Antibody and Protein Reagents

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Human IgG₁ monoclonal antibody made against SARS-CoV-2 spike protein S1 domain (CR3022) Catalog #NBP3-11813 was purchased from Novus Biologicals (Centennial, CO USA). This antibody binds specifically to amino acids 318-510 in the S (spike) domain of the SARS-CoV spike protein as well as the SARS-CoV-2 (COVID-19) spike protein
Human IgG₁ monoclonal antibody made against SARS-CoV-2 nucleoprotein (CR3009) SKU: MAB12434 was obtained from The Native Antigen Company (Kidlington, Oxfordshire, UK). This antibody recognizes and binds the non-linear/conformational epitope of the N protein of SARS-CoV and SARS-CoV-2.
Recombinant SARS-CoV-2 spike protein S1 subunit and recombinant SARS-CoV-2 nucleocapsid protein were purchased from RayBiotech (Atlanta, GA USA).

Vojdani A., Vojdani E., Melgar A.L, & Redd J. (2022). Reaction of SARS-CoV-2 antibodies with other pathogens, vaccines, and food antigens. Frontiers in Immunology, 13, 1003094.

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SARS-CoV-2 Antibody Detection Assay

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Recombinant SARS-CoV-2 S1 receptor-binding domain subunit (RBD) and recombinant SARS-CoV-2 nucleocapsid protein which had been expressed in HEK293 cells were purchased from RayBiotech (Peachtree Corners, GA). Human coronavirus (HCoV-229E) spike protein (S1 subunit, His Tag) and human coronavirus (HCoV-OC43) spike protein (S1 subunit, His Tag), protein were purchased from Sino Biological (Wayne, PA). The recombinant monoclonal anti-SARS-CoV-2 spike glycoprotein S1 antibody (CR3022) was purchased from Abcam (Cambridge, MA). Streptavidin ImmunoCAPs and tetanus toxoid ImmunoCAPs, as well as ImmunoCAPs to measure total IgG, were purchased from Thermo Fisher Scientific (Portage, MI). Galactose-α-1,3-galactose-β-1,4-GlcNAc-HSA (14 atom spacer) was purchased from Dextra (Reading, UK).

Keshavarz B., Wiencek J.R., Workman L.J., Straesser M.D., Muehling L.M., Canderan G., Drago F., Bonham C.A., Sturek J.M., Ramani C., McNamara C.A., Woodfolk J.A., Kadl A., Platts-Mills T.A, & Wilson J.M. (2021). Quantitative Measurement of IgG to Severe Acute Respiratory Syndrome Coronavirus-2 Proteins Using ImmunoCAP. International Archives of Allergy and Immunology, 182(5), 417-424.

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Labeling SARS-CoV-2 Proteins for Analysis

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We have obtained the Recombinant SARS-CoV-2, S1 Subunit Protein (RBD), and Recombinant SARS-CoV-2 Nucleocapsid Protein from Raybiotech (Peachtree Corners GA, cat# 230-30162-1000 and 230-30164-500 correspondingly). The proteins were labeled using Alexa Fluor™ 546 Protein Labeling Kit from Thermo Scientific (cat# A10237) following manufacturer directions except for the RBD that was purified from the dye with Amicon-Ultra 10K cutoff filters (Merck, Millipore cat#UFC201024) instead of the column provided by the kit has a restrictive MW of 50KD (the recombinant RBD protein was 25KDa). The recovery of the protein and labeling efficiency was measured using nanodrop and was about 80% recovery and 0.02 dye molecules per aminoacid. We added 0.5 μg per well of protein.

Calandria J.M., Bhattacharjee S., Maness N.J., Kautzmann M.A., Asatryan A., Gordon W.C., Do K.V., Jun B., Mukherjee P.K., Petasis N.A, & Bazan N.G. (2021). Elovanoids downregulate SARS-CoV-2 cell-entry, canonical mediators and enhance protective signaling in human alveolar cells. Scientific Reports, 11, 12324.

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