Recombinant SARS-CoV-2 S1 receptor-binding domain subunit (RBD) and
recombinant SARS-CoV-2 nucleocapsid protein which had been expressed in HEK293 cells were purchased from RayBiotech (Peachtree Corners, GA). Human coronavirus (HCoV-229E) spike protein (S1 subunit, His Tag) and human coronavirus (HCoV-OC43) spike protein (S1 subunit, His Tag), protein were purchased from Sino Biological (Wayne, PA). The recombinant monoclonal anti-SARS-CoV-2 spike glycoprotein S1 antibody (CR3022) was purchased from Abcam (Cambridge, MA).
Streptavidin ImmunoCAPs and tetanus toxoid ImmunoCAPs, as well as ImmunoCAPs to measure total IgG, were purchased from Thermo Fisher Scientific (Portage, MI). Galactose-α-1,3-galactose-β-1,4-GlcNAc-HSA (14 atom spacer) was purchased from Dextra (Reading, UK).
Keshavarz B., Wiencek J.R., Workman L.J., Straesser M.D., Muehling L.M., Canderan G., Drago F., Bonham C.A., Sturek J.M., Ramani C., McNamara C.A., Woodfolk J.A., Kadl A., Platts-Mills T.A, & Wilson J.M. (2021). Quantitative Measurement of IgG to Severe Acute Respiratory Syndrome Coronavirus-2 Proteins Using ImmunoCAP. International Archives of Allergy and Immunology, 182(5), 417-424.