Monomuls 90 o18

All liquid crystalline nanosystems were prepared from glyceryl monooleate lipid Monomuls^® 90-O18 (1-(cis-9-octadecenoyl)-rac-glycerol), (GMO) (BASF, Ludwigshafen, Germany) and used without further purification. All formulations were prepared in HPLC-grade water.
Resveratrol was acquired from Sigma-Aldrich Chemical Co (St. Louis, MO, USA).
Dichloromethane (POCH) was dried over CaH₂ and distilled under reduced pressure prior to use. THF (POCH) was distilled over a sodium-potassium alloy. Dowex 50WX8 (Sigma-Aldrich, Darmstadt, Germany) was washed with dry THF before use. Methoxy ether poly(ethylene oxide) 5000 (mPEO₁₂₃-OH, TCI, M_n(GPC) = 5 200, M_n(NMR) = 5 400) was dried by two azeotropic distillations using anhydrous toluene. L-lactide (LA) (>99.5%, Forusorb) was purified by sublimation two times before use. 1,8-Diazabicyclo[5.4.0]undec-7-ene (DBU) (98%, Sigma-Aldrich) was distilled under reduced pressure over BaO. Triethylamine (TEA, >99%, Sigma-Aldrich) was distilled under reduced pressure over BaO. 2,2-Bis(hydroxymethyl)propionic acid (bis-MPA, >97%, TCI), ethanol (96%, POCH), ethyl chloroformate (97%, Sigma-Aldrich), Amberlyst 15 (Sigma-Aldrich) and magnesium sulphate (Chempur, Piekary Slaskie, Poland) were used as received.

L-α-phosphatidylcholine hydrogenated (soy) (HSPC) was purchased from Avanti Polar Lipids Inc. (Alabaster, AL, USA) and used without further purification. Surfactant Tween 80 was obtained from Syndesmos S.A. (Athens, Greece), while Span 80 was procured from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). The chloroform, methanol, and H₂O used were of HPLC grade and purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA). In addition, fetal bovine serum (FBS) was obtained from Thermo Fischer Scientific Inc. (Waltham, MA, USA). Quercetin was purchased from Fluka, BioChemika (Buchs, Switzerland). The lipids used for the preparation of the liquid crystalline nanosystems were glyceryl monooleate Monomuls^® 90-O18 [GMO, 1-(cis-9-octadecenoyl)-rac-glycerol], which was purchased from BASF (Ludwigshafen, Germany), and phytantriol (PHYT, 3,7,11,15-tetramethyl-1,2,3-hexadecanetriol), which was purchased from DSM Nutritional Products Ltd. (Heerlen, The Netherlands). Both lipids were used without further purification. As regards the stabilizer, Pluronic^® F-127 (Poloxamer P407) (PEO₉₈-PPO₆₇-PEO₉₈), with an average molar mass of 12,600 g/mol, was purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA).