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Reagent based technique

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Reagent-based technique is a laboratory method used to analyze and detect specific substances or molecules in a sample. It involves the use of chemical reagents that interact with the target analyte, producing a measurable signal or change that can be detected and quantified. This technique provides a standardized and reliable approach for various analytical applications in fields such as chemistry, biology, and diagnostics.

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Lab products found in correlation

Gel dock system, by Bio-Rad (2 mentions) Anti tata binding protein tbp, by Cell Signaling Technology (2 mentions) Polyacrylamide gel, by Bio-Rad (2 mentions) Anti ube3a, by Cell Signaling Technology (2 mentions) Anti α tubulin, by Cell Signaling Technology (2 mentions)

2 protocols using reagent based technique

1

Subcellular Fractionation and Ube3a Quantification

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Nuclear and cytosolic fractions of cortical samples (Extended Data Fig. 2d) were separated using a commercially available reagent-based technique (Fisher Scientific) according to manufacturer instructions. Proteins were loaded onto 4–15% polyacrylamide gels (Bio-Rad), transferred to PVDF membranes, blocked with 5% nonfat milk in TBS-T, and incubated overnight at 4 degrees with the following antibodies; anti-Ube3a (#7526, Cell Signaling), anti-alpha-Tubulin (#3873, Cell Signaling), and anti-TATA-binding protein (TBP; #8515, Cell Signaling). After TBS-T washes, blots were incubated with species-specific HRP-conjugated secondary antibodies for 1–2 hours at room temperature. Blots were developed with Pico chemiluminescent reagent (Pierce) and digital images were acquired using the gel dock system (Bio-Rad). Following subcellular fractionation, nuclear and cytosolic Ube3a levels were normalized to levels of TBP and alpha-Tubulin, respectively.
Krishnan V., Stoppel D.C., Nong Y., Johnson M.A., Nadler M.J., Ozkaynak E., Teng B.L., Nagakura I., Mohammad F., Silva M.A., Peterson S., Cruz T.J., Kasper E.M., Arnaout R, & Anderson M.P. (2017). Autism gene Ube3a and seizures impair sociability by repressing VTA Cbln1. Nature, 543(7646), 507-512.
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2

Subcellular Fractionation and Ube3a Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Nuclear and cytosolic fractions of cortical samples (Extended Data Fig. 2d) were separated using a commercially available reagent-based technique (Fisher Scientific) according to manufacturer instructions. Proteins were loaded onto 4–15% polyacrylamide gels (Bio-Rad), transferred to PVDF membranes, blocked with 5% nonfat milk in TBS-T, and incubated overnight at 4 degrees with the following antibodies; anti-Ube3a (#7526, Cell Signaling), anti-alpha-Tubulin (#3873, Cell Signaling), and anti-TATA-binding protein (TBP; #8515, Cell Signaling). After TBS-T washes, blots were incubated with species-specific HRP-conjugated secondary antibodies for 1–2 hours at room temperature. Blots were developed with Pico chemiluminescent reagent (Pierce) and digital images were acquired using the gel dock system (Bio-Rad). Following subcellular fractionation, nuclear and cytosolic Ube3a levels were normalized to levels of TBP and alpha-Tubulin, respectively.
Krishnan V., Stoppel D.C., Nong Y., Johnson M.A., Nadler M.J., Ozkaynak E., Teng B.L., Nagakura I., Mohammad F., Silva M.A., Peterson S., Cruz T.J., Kasper E.M., Arnaout R, & Anderson M.P. (2017). Autism gene Ube3a and seizures impair sociability by repressing VTA Cbln1. Nature, 543(7646), 507-512.
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