The metal salt, cobalt chloride (CoCl2·6H2O), tryptophane, formaldehyde, HCl, and NaOH were of analytical grade and were purchased from Sigma Aldrich, India. Recombinant human insulin was purchased from Elli Lilly, India. For cell culture, DMEM cell culture media, Fetal Bovine Serum (FBS), 100× penicillin–streptomycin, and phosphate buffer solution (PBS) having pH 7.4 were purchased from HiMedia, India. Human Primary Epithelial Keratinocytes (HEKa cells) ATCC-PCS-200-011 were procured from Himedia, India, cultured, maintained, and treated in DMEM containing 5% FBS at 37 °C and 5% CO2.
Dmem cell culture media
Manufactured by HiMedia
Sourced in India
DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture media used to support the growth and maintenance of various cell types in vitro. It provides the necessary nutrients, vitamins, and growth factors required for cell proliferation and survival. The core function of DMEM is to create an optimal environment for the cultivation of cells, facilitating their attachment, growth, and differentiation.
Automatically generated - may contain errors
Lab products found in correlation
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Formaldehyde, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
Fetal bovine serum (fbs), by HiMedia (1 mentions)
Cobalt chloride, by Merck Group (1 mentions)
Tryptophane, by Merck Group (1 mentions)
Pb no3 2, by Loba Chemie (1 mentions)
2 protocols using dmem cell culture media
1
Cobalt-Induced Keratinocyte Proliferation
2
Insulin Cytotoxicity Assay Protocol
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All the metal salts of analytical grade, including NiSO4 (nickel sulphate) and Pb(NO3)2 (lead nitrate) were purchased from Loba Chemie, India. Recombinant human insulin was purchased from (Elli Lilly, India). DMEM cell culture media, fetal bovine serum (FBS), penicillin–streptomycin were purchased from HiMedia, India. The rest of the chemicals were purchased from HiMedia and were of analytical grade.
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