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Gpc sec software version 1.2

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The GPC/SEC Software (version 1.2) is a data analysis software designed for Gel Permeation Chromatography (GPC) and Size Exclusion Chromatography (SEC) applications. The software provides tools for data processing, analysis, and reporting of chromatographic results.

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Lab products found in correlation

Gpc system, by Waters Corporation (2 mentions) 1260 isopump, by Agilent Technologies (1 mentions) Pl gel 5 μm mixed c columns, by Agilent Technologies (1 mentions) 1260 als autosampler, by Agilent Technologies (1 mentions) La 960, by Horiba (1 mentions)

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GPC/SEC software (24 citations) GPC software (4 citations)

4 protocols using gpc sec software version 1.2

1

Molecular Characterization of PDEA Copolymers

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The molecular weights
and polydispersities of the PDEA macro-CTA and PDEA–P(MAA-stat-BzMA) diblock copolymers were determined by GPC using
THF eluent. The GPC setup consisted of two 5 μm (30 cm) Mixed
C columns and a WellChrom K-2301 refractive index detector operating
at 950 ± 30 nm. The mobile phase contained 2.0% v/v triethylamine
and 0.05% w/v butylhydroxytoluene (BHT) with a toluene flow
rate marker, and the flow rate was fixed at 1.0 mL min–1. Copolymer solutions (1.0% w/v) were prepared in THF. A series of
ten near-monodisperse poly(methyl methacrylate) standards (Mp values ranging from 1280 to 330 000
g mol–1) were used for calibration. Data were analyzed
using Agilent GPC/SEC software version 1.2.
Canning S.L., Neal T.J, & Armes S.P. (2017). pH-Responsive Schizophrenic Diblock Copolymers Prepared by Polymerization-Induced Self-Assembly. Macromolecules, 50(16), 6108-6116.
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2

Polymer Molecular Weight Analysis via GPC

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Polymer molecular
weights and polydispersities were determined using a DMF GPC instrument
operating at 60 °C that comprised two Polymer Laboratories PL
gel 5 μm Mixed C columns and one PL gel 5 μm guard column
connected in series to an Agilent Technologies 1260 Infinity multidetector
suite (refractive index detector only) and an Agilent Technologies
1260 ISO pump fitted with a 1260 ALS autosampler. The GPC eluent was
HPLC-grade DMF containing 10 mM LiBr and was filtered prior to use.
The flow rate used was 1.0 mL min–1 and DMSO was
used as a flow-rate marker. Calibration was conducted using a series
of 10 near-monodisperse poly(methyl methacrylate) standards (Mn = 625–618000 g mol–1, K = 2.094 × 10–3, α
= 0.642). Chromatograms were analyzed using Agilent Technologies GPC/SEC
software version 1.2.
Ratcliffe L.P., Couchon C., Armes S.P, & Paulusse J.M. (2016). Inducing an Order–Order Morphological Transition via Chemical Degradation of Amphiphilic Diblock Copolymer Nano-Objects. Biomacromolecules, 17(6), 2277-2283.
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3

Characterizing Latex Polymer Distributions

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The molecular weight distributions of the latex samples were measured by gel permeation chromatography (GPC). For GPC measurement, dried latex samples were dissolved in tetrahydrofuran (THF) at a concentration of 5 mg/ml. A Waters GPC system consisted of two columns with exclusion limits of 2.0 × 107 and 5.0 × 104, and a differential refractive index detector was applied to the samples. THF was used as the mobile phase at a flow rate of 0.5 ml/min. Commercially obtained polyisoprene standards of known molecular weight were used to calibrate the columns. The measurement temperature was set at 35 °C, and the injection volume was 10 μl. MW information of each latex sample was collected and analyzed using Agilent (Santa Clara, USA) GPC/SEC Software (version 1.2). The mean and standard deviation of latex MW were calculated from three biological replicates.
The sizes and distributions of RPs in each latex sample were determined by an LA-960 (HORIBA Scientific, Japan) Light Scattering Particle Size Distribution Analyzer at 30 °C with a 90° determination angle.
Xin S., Hua Y., Li J., Dai X., Yang X., Udayabhanu J., Huang H, & Huang T. (2021). Comparative analysis of latex transcriptomes reveals the potential mechanisms underlying rubber molecular weight variations between the Hevea brasiliensis clones RRIM600 and Reyan7-33–97. BMC Plant Biology, 21, 244.
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4

Latex Molecular Weight and Particle Size Analysis

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The weight distributions of the latex samples were measured by gel permeation chromatography (GPC). For GPC measurement, dried latex samples were dissolved in tetrahydrofuran (THF) at a concentration of 5 mg/ml. A Waters GPC system consisted of two columns with exclusion limits of 2.0×10 7 and 5.0×10 4 , and a differential refractive index detector was applied to the samples. THF was used as the mobile phase at a ow rate of 0.5 ml/min. Commercially obtained polyisoprene standards of known molecular weight were used to calibrate the columns. The measurement temperature was set at 35 °C, and the injection volume was 10 ml. MW information of each latex sample was collected and analyzed using Agilent (Santa Clara, USA) GPC/SEC Software (version 1.2). The mean and standard deviation of latex MW were calculated from three biological replicates.
The sizes and distributions of RPs in each latex sample were determined by an LA-960 (HORIBA Scienti c, Japan) Light Scattering Particle Size Distribution Analyzer at 30 °C with a 90° determination angle.
Xin S., Hua Y., Li J., Dai X., Yang X., Udayabhanu J., Huasun H., & Huang T. (2021). Comparative analysis of latex transcriptomes reveals the potential mechanisms underlying rubber molecular weight variations between the Hevea brasiliensis clones RRIM600 and Reyan7-33-97.
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