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Phosphate buffered saline a 3 4 5 dimethylthiazol 2 yl 5 3 carboxymethoxyphenyl 2 4 sulfophenyl 2h tetrazolium mts

Manufactured by Promega
Sourced in United States

Phosphate-buffered saline (PBS) is a common buffer solution used in biological research. It is an aqueous solution containing sodium phosphate and sodium chloride. MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) is a tetrazolium compound used in cell viability assays to measure the metabolic activity of cells.

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2 protocols using phosphate buffered saline a 3 4 5 dimethylthiazol 2 yl 5 3 carboxymethoxyphenyl 2 4 sulfophenyl 2h tetrazolium mts

1

Cultured Cell Line Experiments

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HUVECs, NHEKs, NHDFs, and related media for cell cultures, including endothelial growth medium-2 (EGM-2), keratinocyte growth medium-2 (KGM™-2), and fibroblast growth medium (FGM™) were purchased from Lonza (Walkersville, MD, USA). Cells were incubated at 37 °C and 5% CO2 throughout the experiment. Phosphate-buffered saline (PBS), a 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) (Promega) (Madison, WI, USA) colorimetric assay, and growth factor reduced matrigel BD (Corning), (New York, NY, USA) were purchased from Sigma-Aldrich (Milwaukee, WI, USA).
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2

Cultured Cell Line Experiments

Check if the same lab product or an alternative is used in the 5 most similar protocols
HUVECs, NHEKs, NHDFs, and related media for cell cultures, including endothelial growth medium-2 (EGM-2), keratinocyte growth medium-2 (KGM™-2), and fibroblast growth medium (FGM™) were purchased from Lonza (Walkersville, MD, USA). Cells were incubated at 37 °C and 5% CO2 throughout the experiment. Phosphate-buffered saline (PBS), a 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) (Promega) (Madison, WI, USA) colorimetric assay, and growth factor reduced matrigel BD (Corning), (New York, NY, USA) were purchased from Sigma-Aldrich (Milwaukee, WI, USA).
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