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6 protocols using acetonitrile mecn

1

Purification and Characterization of RVR

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RVR (98%) was purchased from Sigma Aldrich as pure RVR-I and used without further purification. RVR-II was produced pure by slow evaporation from ethyl acetate solutions. All solvents used were of analytical grade and used as received. Acetonitrile (MeCN, ≥99.9%) and 2-propanol (IPA, ≥99.5%) were purchased from Honeywell Research Chemicals, acetone (Ace, ≥99.8%) from Fisher Scientific UK Ltd, and ethyl acetate (EA, 99.8%) from Sigma Aldrich.
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2

Analytical Reagent Procurement for Research

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Methanol (MeOH) and acetonitrile (MeCN) were purchased from Honeywell (Muskegon, MI, USA). Ammonium formate (≥ 99.0%) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Primary-Secondary-Amine (PSA bonded silica) was purchased from Supelco (Bellefonte, PA, USA). C 18 end-capped resin was purchased from Agilent (Santa Clara, CA, USA). Formic acid (99.0%, GR) was purchased from Samchun Chemical Co. (Gyeonggi-do, Korea). Formic acid (LiChropur ® ) was purchased from Merck (Darmstadt, Germany). Other chemicals used in this study were analytical grades.
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3

Fmoc-Protected Amino Acid Synthesis

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Fmoc-protected amino acids were purchased from CEM Corporation, and Pepceuticals. N, N-Dimethylformamide (DMF) and diethyl ether (Et2O) were purchased from Rathburn. Triisopropylsilane (TIPS), Trifluoroacetic acid (TFA), N, N-Diisopropylethylamine (DIPEA), N,N’-diisopropylcarbodiimide (DIC), ethyl cyano (hydroxyimino) acetate (Oxyma Pure), fluorescein-5-isothiocyanate (FITC), Fmoc-Lys(Alloc)-OH, Tetrakis(triphenylphosphine)palladium(0) {Pd(PPh3)4)}, and phenylsilane were purchased from Fluorochem. Morpholine was purchased from Alfa Aesar. Dichloromethane (DCM) was purchased from VWR. Acetonitrile (MeCN) was purchased from Honeywell. TentaGel S RAM resin was purchased from Rapp Polymere. All other reagents were purchased from Sigma Aldrich.
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4

Ginseng Ginsenoside Profiling Protocol

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The ginsenoside mixture of Rg1, Re, Rf, Rg2, Rb1, Rc, Rb2, and Rd (0.1 g/L) was purchased from Cerilliant Co. (Round Rock, TX, USA), and ginsenoside Ro were purchased from Embo Laboratory (Daejeon, Korea). Voucher specimens of ginseng samples were deposited to Phytobean AC LTD (Yecheon-gun, Korea). HPLC-grade methanol (MeOH), water, and acetonitrile (MeCN) were purchased from Honeywell (Morris Plains, NJ, USA). Analytical grade phosphoric acid was purchased from Sigma-Aldrich (St. Louis, MO, USA).
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5

Quantification of Mycotoxin Standards

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Mycotoxin standards—DON, ZEN, T-2, and HT-2 toxins—were obtained from Romer Lab (Cheshire, UK). LC-MS grade methanol (MeOH) and acetonitrile (MeCN) were purchased from Honeywell (London, UK). Ultrapure water (resistivity of 18.2 MΩ-cm) was obtained from a Milli-Q system (Millipore, Burlington, MA, USA). LC-MS grade formic acid and syringe filters (PTFE, 0.22 μm pore size) were supplied by Sigma-Aldrich (St. Louis, MO, USA) and Waters (Wilmslow, UK), respectively.
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6

Analytical Reagents for Chromatography

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Methanol (MeOH) and acetonitrile (MeCN) were obtained from Honeywell (Muskegon, MI, USA) at HPLC grade. Ammonium formate (≥99.0%) and potassium phosphate dibasic (≥98.0%, ACS reagent) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Formic acid was obtained from Merck (Darmstadt, Germany) with an available purity of 99.0%. Hydrochloric acid (HCl, 36.0~38.0%, electronic grade) was obtained from Duksan pure Chemicals (Ansan, Korea). Sodium chloride, anhydrous Na2SO4, trisodium citrate, disodium citrate, anhydrous MgSO4, primary secondary amine (PSA), octadecyl silane (C18), and graphitized carbon black (GCB) were purchased from Agilent (Santa Clara, CA, USA).
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