Mass spectrophotometer

Melting points of the two compounds were determined using a Bock monoscope and were uncorrected. UV spectra were measured on a UNCAM UV/Vis spectrophotometer (Newington, USA). Mass spectra were determined on a Jeol mass spectrophotometer (Tokyo, Japan). ¹H and ¹³C NMR spectra were obtained on Bruker DRX-300 spectrophotometer (Fallanden, Switzerland) with tetramethylsilane as an internal standard, and the heteronuclear multiple bond correlation (HMBC) was measured using a standard pulse sequence. High-performance liquid chromatography (HPLC) was carried out using a Shimadzu model LC-8A. The circular dichroism (CD) spectrum was done at the Department of Pharmacognosy, University of Mississippi, USA. TLC, column chromatography, and paper chromatography (PC) were performed on precoated Si GF²⁵⁶, Si gel (60–120 Mesh, Merck India), Sephadex LH-20 (Sigma, USA), and Whatman paper to characterize compounds 1 and 2.

Melting points (m. p.) of compounds 1 and 2 were determined using a Bockmonoscope. A UNCAM UV/vis spectrophotometer (Newington, United States) was used to record UV spectra. Mass spectra were determined on a Jeol mass spectrophotometer (Tokyo, Japan). ¹H and ¹³C NMR spectra were obtained on Bruker DRX-300 spectrophotometer (Fallanden, Switzerland) with tetramethyl silane as an internal standard, and the heteronuclear multiple bond correlation (HMBC) was measured using a standard pulse sequence. High-performance liquid chromatography (HPLC) was performed using a Shimadzu model LC-8A. To get the circular dichroism (CD) spectrum, the facility at the Department of Pharmacognosy, University of Mississippi, United States, was used. The spectrums for both compounds are presented in the (Supplementary Figure S2). TLC, column chromatography, and paper chromatography (PC) were performed on precoated Si GF²⁵⁶, Si gel (60–120 Mesh, Merck India), Sephadex LH-20 (Sigma, United States ), and Whatman paper to characterize both compounds 1 and 2.