Phantom v4

Manufactured by Ametek

The Phantom v4.2 is a high-speed camera designed for advanced imaging applications. It features a 4-megapixel CMOS sensor and can capture images at frame rates up to 6,400 fps at full resolution. The camera supports multiple recording modes and image resolutions to suit various experimental requirements.

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Lab products found in correlation

Image processing toolbox, by MathWorks (1 mentions) Phantom miro m310, by Ametek (1 mentions)

Close spelling variants of this product

Phantom v4.2 camera (7 citations)

4 protocols using phantom v4

Automated Fluorescent Microscopy Imaging

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Fluorescent and high imaging was accomplished using an automated Nikon TiE inverted microscope with a Retiga 2000R monochromatic camera as well as a Vision Research Phantom v4.2 high speed monochromatic camera.

Martel J.M., Smith K.C., Dlamini M., Pletcher K., Yang J., Karabacak M., Haber D.A., Kapur R, & Toner M. (2015). Continuous Flow Microfluidic Bioparticle Concentrator. Scientific Reports, 5, 11300.

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Kinematic Analysis of Rat Head Impact Using FEM

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One animal was euthanized and used for determining the CHI model kinematic parameters. Rat skull was exposed and 3 round head pins were attached to the skull (one near the nose and two near the ears) as photo targets. The animal was positioned under the impactor and the head was struck similar to what was described earlier (20° angle and 2 or 5 mm displacement). The positions of the photo targets and their image in a mirror that was placed in front of the animal were recorded using a high speed camera (Phantom, v4.2, Vision Research, Inc., NJ) at 2200 frames per second. The three-dimensional positions of the photo targets were calculated using and in-house code in Matlab and the image processing toolbox (Mathworks, MA). A finite element (FE) model of the rat head with the attached photo targets was developed in LS-Dyna (LSTC, CA). The displacements of the photo targets were given as inputs to the FE model and based on motion of the head center of gravity, the time histories and peak values of the resultant linear and rotational velocities and accelerations of the head were determined. The value of HIC15 (the head injury criterion with 15 ms maximum time interval) was also calculated for the resultant linear acceleration, which is an indication of the intensity of the head acceleration.

Tyburski A.L., Cheng L., Assari S., Darvish K, & Elliott M.B. (2017). Frequent mild head injury promotes trigeminal sensitivity concomitant with microglial proliferation, astrocytosis, and increased neuropeptide levels in the trigeminal pain system. The Journal of Headache and Pain, 18(1), 16.

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High-Speed Microscopy for Microfluidic Flow

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Epifluorescence and brightfield images were taken using an inverted microscope (Axio Observer.A1, Zeiss; 10x and 20x objectives) connected to a cooled interline CCD camera (Clara, Andor). Videos of microfluidic flow experiments were taken using high-speed cameras (Phantom v4.2 and Phantom Miro M310, Vision Research) at frame rates ranging from 2000-8500fps and exposure times ranging from 2-10 μs.

Chen L., An H.Z., Haghgooie R., Shank A.T., Martel J.M., Toner M, & Doyle P.S. (2016). Flexible Octopus-shaped Hydrogel Particles for Specific Cell Capture. Small (Weinheim an der Bergstrasse, Germany), 12(15), 2001-2008.

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Aortic Valve Hemodynamics Evaluation

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The ViVitro LHS was used as a pulse duplicator with capabilities of measuring flow, pressure, and cardiac output. The LHS consisted of a piston pump system, various adapters for tunable afterload generation, an adjustable left ventricular membrane, flow and pressure monitoring systems, a waveform generator, and a data acquisition system. In addition, an adjustable jig was used to attach the repaired aortic roots to the system. A high-speed video camera (Phantom V4.2, Vision Research, Wayne, NJ) was connected to an endoscope and light source to record images of valvular cusp motion from the aortic side (Figure 1,D). Further details have been described by our group. 14 For valve hemodynamic measurements, the control or intervention aortic root was connected at room temperature to the LHS. The system contained 0.9% normal saline (density of 0.9 g/mL and a viscosity of 1 mPa-s) as the fluid medium, which was pressurized from 0 mm Hg to a systolic pressure of 120 mm Hg and a diastolic pressure of 80 mm Hg. The heart rate was set at 70 beats/min, and cardiac output was set at 3 L/min for all experiments. Additional parameters measured include regurgitant volume and fraction, and effective regurgitant orifice (ERO) area.

Al-Atassi T., Toeg H.D., Jafar R., Sohmer B., Labrosse M, & Boodhwani M. (2015). Impact of aortic annular geometry on aortic valve insufficiency: Insights from a preclinical, ex vivo, porcine model. The Journal of thoracic and cardiovascular surgery, 150(3).

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