Proteins were resolved on 10% SDS–PAGE gels, transferred to nitrocellulose membranes, blocked with 5% nonfat dry milk in TBS/0.1% Tween20 (TBS-T), washed three times in TBS-T, and incubated overnight in primary antibodies in 5% BSA/TBS (BSA cat. no. A7906; Sigma Aldrich) at 4°C. Antibody dilutions were as follows: rabbit Cx43 (Cell Signaling), 1:2000; mouse ZO-1 (Invitrogen), 1:1000; mouse clathrin heavy chain (BD Transduction), 1:2000; α-tubulin (cat. no. T2096, Sigma-Aldrich), 1:5000. Blots were washed three times in TBS-T and incubated in the appropriate secondary antibodies (goat anti-mouse-HRP and goat anti-rabbit HRP, cat. nos. G21040 and G21234, respectively; Invitrogen) for 30 min at 1:3000 dilution at RT. Relevant proteins were detected with enhanced chemiluminescent reagent. ImageJ (Nationl Institutes of Health) was used to quantify protein band intensities.
Rabbit cx43
Manufactured by Cell Signaling Technology
Sourced in United States
Rabbit Cx43 is an antibody product developed by Cell Signaling Technology. It is designed to detect connexin 43, a protein that forms gap junctions between cells and facilitates intercellular communication.
Automatically generated - may contain errors
Lab products found in correlation
Mouse zo 1, by Thermo Fisher Scientific (1 mentions)
α tubulin, by BD (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Mouse β actin, by Cell Signaling Technology (1 mentions)
Normal rabbit igg, by Cell Signaling Technology (1 mentions)
Il 18, by Abcam (1 mentions)
Alexa fluor 488 donkey anti mouse, by Thermo Fisher Scientific (1 mentions)
Interleukin 2 (il 2), by Abcam (1 mentions)
Lucifer yellow, by Merck Group (1 mentions)
Protein a g plus agarose, by Santa Cruz Biotechnology (1 mentions)
Hoechst 33342, by Dojindo Laboratories (1 mentions)
Interleukin 6 (il 6), by Abcam (1 mentions)
Rabbit ubiquitin, by Cell Signaling Technology (1 mentions)
2 protocols using rabbit cx43
1
Western Blot Analysis of Proteins
2
Rg1 Bioactivity and Signaling Pathway
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Rg1 (PubChem CID: 441923, C42H72O14, ≥ 98%) was purchased from Jilin University (Changchun, Jilin Province, China). Lucifer Yellow was obtained from Sigma-Aldrich (St. Louis, MO, United States). High-glucose Dulbecco’s Modified Eagle Media (DMEM) and fetal bovine serum were produced by Gibco (Grand Island, NY, United States). Additional antibody sources: mouse β-actin, normal rabbit IgG, rabbit Cx43, mouse Cx43, rabbit ubiquitin, and mouse ubiquitin antibodies were obtained from Cell Signaling Technology (Beverly, MA, United States); Alexa Fluor-488 donkey anti-mouse and 546 donkey anti-rabbit secondary antibodies were produced by Invitrogen (Carlsbad, CA, United States). Protein A/G Plus-Agarose was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, United States). Hoechst 33342 was produced by Dojindo Laboratories (Kumamoto, Japan). Rat and cell supernatant enzyme-linked immunosorbent assay (ELISA) Kits for interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), caspase-1, IL-2, IL-6, and IL-18 were purchased from Abcam (Cambridge, United Kingdom).
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