Penicillin streptomycin stock solution
Penicillin/streptomycin stock solution is a commonly used antibiotic mixture for cell culture applications. It provides a combination of the antibiotics penicillin and streptomycin in a concentrated liquid form.
Lab products found in correlation
14 protocols using penicillin streptomycin stock solution
Prostate Cancer Cell Culture Conditions
SILAC Labeling of Breast Cancer Cells
Plasmid Transfection and Cell Culture
Culturing Human Multiple Myeloma Cell Lines
HDL-c Isolation via KBr Ultracentrifugation
Hypoxia-Reoxygenation Injury Model in Murine Renal Tubular Cells
Lats2 was overexpressed via transfecting with a lentivirus incorporating the Lats2 recombinant plasmid (Ubi-MCS-3FLAG-CBh-gcGFP-IRES-puromycin, GENECHEM, Shanghai, China), referred to as LV-Lats2. LV-NC, a lentivirus incorporating an empty plasmid, served as a negative control. Viral particles were removed 16 h later after transfection, and cells were replenished with fresh TEC medium. Puromycin selection commenced 24 h later with the addition of 2 μg/mL puromycin. Surviving cells were isolated into single cell clones. The cells after treatment were lysed in RIPA lysis buffer (Beyotime, Shanghai, China) for western blotting analysis.
Xenopus Oocyte Preparation and Maintenance
Cytokine Release Assay for Immune Cells
Isolation and Culture of Murine Splenocytes and Peritoneal Macrophages
Resident peritoneal macrophages were harvested by instilling and withdrawing 10 ml of complete cell culture medium: RPMI 1640 medium supplemented with 2 mM L-glutamine, 5% heat-inactivated fetal bovine serum, and 1% penicillin-streptomycin stock solution (Gibco, Barcelona, Spain). Cells were washed once with the same medium, and plated at a density of 1.5 x 105 cells in 200 μl of medium per well, in a 96 well tissue culture plate. Peritoneal macrophages were allowed to adhere for 5 h at 37°C in a 5 % CO2 atmosphere, the non-adherent cells were removed, and the adherent macrophages were cultured for 72 h prior to be challenged with the indicated stimuli.
Lipoprotein Isolation and Purification
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!