Kg 1 cells

Manufactured by American Type Culture Collection

Sourced in Canada, United States

KG-1 cells are a human acute myelogenous leukemia (AML) cell line. They are suspension cells derived from the bone marrow of a 59-year-old male with AML. KG-1 cells are used for research purposes in the study of AML and related hematological disorders.

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Lab products found in correlation

Pen strep, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Iscove modified dulbecco media (imdm), by Thermo Fisher Scientific (1 mentions) Gentamicin sulfate, by Thermo Fisher Scientific (1 mentions) Sf 900 3 media, by Thermo Fisher Scientific (1 mentions) Venetoclax abt 199, by Selleck Chemicals (1 mentions) Annexin 5 apc, by BioLegend (1 mentions) Facscanto flow cytometry, by BD (1 mentions) Celltiter glo luminescent cell viability assay, by Promega (1 mentions) Ponatinib ap24534, by Selleck Chemicals (1 mentions) Molm 13 cells, by Leibniz Institute DSMZ (1 mentions)

4 protocols using kg 1 cells

Cell Culture Conditions for Various Cell Lines

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SKW3 T cells (DSMZ) were cultured in RPMI-1640+GluMax (Thermo Fisher Scientific) complemented with 10% fetal bovine serum (FBS, Sigma-Aldrich), 10 mM HEPES and 50 U/mL Pen-Strep (Thermo Fisher Scientific) at 37 °C and 5% CO₂.
LentiX cells and 293T cells were cultured in DMEM (Thermo Fisher Scientific) supplemented with 10% FBS, 2 mM L-Glutamine, 10 mM HEPES and 50 U/mL Pen-Strep (Thermo Fisher Scientific) at 37 °C and 5% CO₂.
KG-1 cells (ATCC) were cultured in IMDM (Thermo Fisher Scientific) supplemented with 10% FBS and 50 U/mL Pen-Strep (Thermo Fisher Scientific) at 37 °C and 5% CO₂.
SF9 cells were cultured in SF900-III media (Thermo Fisher) supplemented with 10% FBS and 10 mg/mL gentamicin sulfate (Thermo Fisher) at 27 °C and atmospheric CO₂.
Hi5 cells were grown in insect cell culture medium (Expression Systems) supplemented with 10 mg/mL gentamicin sulfate (Thermo Fisher) at 27 °C and atmospheric CO₂.
Jurkat cell lines were cultured in RPMI 1640 supplemented with 10% FBS, 2 mM L-Glutamine, 50 U/mL Penicillin, 50 μg/mL Streptomycin, and 50 μM β-mercaptoethanol at 37 °C and 5% CO₂.
HEK293T cell line was cultured in DMEM supplemented with 10% FBS, 2 mM L-Glutamine, and 18 mM HEPES at 37 °C and 5% CO₂.

Zhao X., Kolawole E.M., Chan W., Feng Y., Yang X., Gee M., Jude K.M., Sibener L.V., Fordyce P.M., Germain R.N., Evavold B.D, & Garcia K.C. (2022). Tuning T cell receptor sensitivity through catch bond engineering. Science (New York, N.Y.), 376(6589), eabl5282.

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Comprehensive Cell Line Characterization

Cited 2 times

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KG1 cells were purchased from ATCC. Murine cell lines BBC1 BBC2, ZNF112, and CEP2A, were generated in-house^{8 (link)–10 (link)}. The TKI resistant cell lines BBC1-R, ZNF112-R, CEP2A-R and KG-1R were generated as described previously^{7 (link)}. All cell lines were cultured in RPMI 1640 medium containing 10% FBS, 100 U/mL penicillin, and 100 U/mL streptomycin. Cells were routinely checked by morphology and western blot confirmation of FGFR1 fusion kinase. All of these cell lines were free of mycoplasma contamination.
Drugs used in these studies were: BGJ398, Ponatinib (AP24534), and Venetoclax (ABT199) (Selleckchem, Houston, TX). For drug inhibition assays, cells were treated at the indicated concentrations of BGJ398, Ponatinib or ABT199 for 72 h, followed by cell viability assays using the CellTiter-Glo Luminescent Cell Viability Assay (Promega, Madison, WI). To measure apoptosis, 10⁶ cells were treated with the indicated concentration of drugs for 24 h and then stained with APC Annexin V and DNA binding dye 7-amino-actinomycin (7-AAD) according to the manufacturer’s protocol (Biolegend, San Diego, CA.) and analyzed using BD FACSCanto flow cytometry (BD Bioscience, San Jose, CA). All drug treatment experiments were repeated at least three times, and the representative results were presented.

Liu Y., Cai B., Chong Y., Zhang H., Kemp C.A., Lu S., Chang C.S., Ren M., Cowell J.K, & Hu T. (2020). Downregulation of PUMA underlies resistance to FGFR1 inhibitors in the stem cell leukemia/lymphoma syndrome. Cell Death & Disease, 11(10), 884.

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Culturing Hematopoietic Cell Lines and MSCs

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OCI-AML3 cells were kindly provided by Dr. M. Minden (Ontario Cancer Institute, Ontario, Canada), KG-1 cells were purchased from the American Type Culture Collection (Manassas, VA, USA), and Molm13 cells were obtained from the German Collection of Microorganisms and Cell Cultures (Braunschweig, Germany). Cells were cultured in RPMI 1640 medium supplemented with 10% heat-inactivated fetal calf serum (FSC), 2 mM L-glutamine, 100 U/ml penicillin, and 100 g/ml streptomycin. MSCs were isolated from BM of healthy subjects as previously described [21 ].

Mak P.Y., Mak D.H., Mu H., Shi Y., Ruvolo P., Ruvolo V., Jacamo R., Burks J.K., Wei W., Huang X., Kornblau S.M., Andreeff M, & Carter B.Z. (2014). Apoptosis repressor with caspase recruitment domain is regulated by MAPK/PI3K and confers drug resistance and survival advantage to AML. Apoptosis : an international journal on programmed cell death, 19(4), 698-707.

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Bone Marrow Samples Analysis in AML

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Bone marrow samples were obtained from 62 patients diagnosed with AML (38 males and 24 females, aged 10–52 years with a mean age of 31.98 ± 9.98 years) by the morphologic, immunologic, cytogenetic and molecular biologic classification in the Department of Hematology of The First Affiliated Hospital of Zhengzhou University (Zhengzhou, Henan Province, China) from September 2014 to September 2017. In addition, bone marrow samples were also collected from 20 healthy people and used as the control. According to French-American-British classification systems, there were 16 cases of M₂, 24 of M₃, 12 of M₄, and 10 of M₅. Complete blood counts revealed that the white blood cell count was 11.2–96.0 × 10⁹/L, platelet count was 31.2–86.1 × 10⁹/L, and hemoglobin level was 5.22–11.97 g/dL. Percentage of immature myeloid cells in bone marrow was 13.44–75.50%. Bone marrow mononuclear cells (BMNCs) were separated from bone marrow samples.
KG-1 cells (American Type Culture Collection, Manassas, VA, USA) were cultured in RPMI 1640 medium at 37 °C with 5% CO₂. Cell growth was observed daily with an inverted microscope, and cells were passaged once every 2–3 days (Kobayashi et al. 2020).

Wang C., Li L., Li M., Wang W., Liu Y, & Wang S. (2020). Silencing long non-coding RNA XIST suppresses drug resistance in acute myeloid leukemia through down-regulation of MYC by elevating microRNA-29a expression. Molecular Medicine, 26, 114.

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