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3 4 5 dimethylthiazol 2 yl 5 3 carboxymethoxyphenyl 2 4 sulfophenyl tetrazolium mts reagent

Manufactured by Promega
Sourced in United States

The 3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-tetrazolium (MTS) reagent is a colorimetric assay for determining the number of viable cells in a cell proliferation or cytotoxicity assay. The MTS reagent is reduced by metabolically active cells, producing a colored formazan product that can be quantified using a spectrophotometer.

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2 protocols using 3 4 5 dimethylthiazol 2 yl 5 3 carboxymethoxyphenyl 2 4 sulfophenyl tetrazolium mts reagent

1

Lipid-based Nanoparticle Formulation

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1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DSPE-PEG), cholesterol, and dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). ICG was purchased from BioActs (Seoul, Korea). 3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-tetrazolium (MTS) reagent was obtained from Promega (Madison, WI, USA). Dulbecco's modified Eagle's medium (DMEM) and Ellma's reagent were obtained from Thermo Scientific (Waltham, MA, USA). All other chemicals were obtained from commercial suppliers, unless otherwise specified.
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2

Multifunctional Theranostic Nanoparticle Synthesis

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1,2-distearoyl-sn-glycero-3 phosphoethanolamine-N-[methoxy (polyethylene glycol) - 2000] amine (DSPE-PEG-NH2) and dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) were purchased from Avanti polar lipid (AL, USA). ICG was purchased from BioActs (Korea). Branched polyethyleneimine (1800 Da) was purchased from Polysciences Inc. (USA). Methanol and dimethyl sulfoxide (DMSO) were obtained from Merck (Germany). 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC), L-glutathione reduced (GSH), N-hydroxysuccinimide (NHS), lithocholic acid and L-buthionine sulfoximine (BSO) were purchased from Sigma (St. Louis, MO). 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4sulfophenyl)- tetrazolium (MTS) reagent was obtained from Promega (Madison, WI, USA). Propidium iodide (PI), fluorescein diacetate (FDA) and Dulbecco's modified Eagle's medium (DMEM) Ellman's reagent were obtained from Thermo Scientific (Waltham, MA, USA). All other chemicals were obtained from commercial suppliers unless otherwise specified.
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