3 4 5 dimethylthiazol 2 yl 5 3 carboxymethoxyphenyl 2 4 sulfophenyl tetrazolium mts reagent

1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DSPE-PEG), cholesterol, and dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). ICG was purchased from BioActs (Seoul, Korea). 3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-tetrazolium (MTS) reagent was obtained from Promega (Madison, WI, USA). Dulbecco's modified Eagle's medium (DMEM) and Ellma's reagent were obtained from Thermo Scientific (Waltham, MA, USA). All other chemicals were obtained from commercial suppliers, unless otherwise specified.

1,2-distearoyl-sn-glycero-3 phosphoethanolamine-N-[methoxy (polyethylene glycol) - 2000] amine (DSPE-PEG-NH₂) and dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) were purchased from Avanti polar lipid (AL, USA). ICG was purchased from BioActs (Korea). Branched polyethyleneimine (1800 Da) was purchased from Polysciences Inc. (USA). Methanol and dimethyl sulfoxide (DMSO) were obtained from Merck (Germany). 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC), L-glutathione reduced (GSH), N-hydroxysuccinimide (NHS), lithocholic acid and L-buthionine sulfoximine (BSO) were purchased from Sigma (St. Louis, MO). 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4sulfophenyl)- tetrazolium (MTS) reagent was obtained from Promega (Madison, WI, USA). Propidium iodide (PI), fluorescein diacetate (FDA) and Dulbecco's modified Eagle's medium (DMEM) Ellman's reagent were obtained from Thermo Scientific (Waltham, MA, USA). All other chemicals were obtained from commercial suppliers unless otherwise specified.