Transfection was done on astrocytes, in 80% confluent T25 flasks using RNAi MAX (Invitrogen) as per the manufacturer's protocol. 62.5 nM of Syn‐hsa‐miR‐92b‐5p miScript miRNA mimic (Qiagen, Cat# MSY0004792) was used for mimic transfection. All‐Stars Negative Control siRNA (Qiagen, Cat# SI03650318) was used as mimic control. miRNA inhibition was done using 62.5 nM of the miRCURY LNA miRNA inhibitor against has‐miR‐92b‐5p (Qiagen, Cat# YI04104160‐ADAFr), and miRCURY LNA miRNA inhibitor control (Qiagen, Cat# YI00199006‐ADA) was used as the inhibitor control. Transfections were done using Opti‐MEM media (Gibco, Cat# 31985–070) for 5 h and replaced with complete MEM. Cells were processed after 48 h of transfection for RNA and protein analysis. Effective doses of mimic and inhibitor miRNA were standardized via qPCR and protein studies.
Syn hsa mir 92b 5p miscript mirna mimic
Manufactured by Qiagen
Sourced in Germany
The Syn-hsa-miR-92b-5p miScript miRNA mimic is a synthetic RNA molecule designed to mimic the function of the human microRNA hsa-miR-92b-5p. MicroRNAs are small, non-coding RNA molecules that play a role in regulating gene expression.
Automatically generated - may contain errors
3 protocols using syn hsa mir 92b 5p miscript mirna mimic
1
Astrocyte miR-92b-5p Modulation
2
miR-92b-5p Transfection in Astroglia
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Transfection was done on astroglia, in 80% con uent T25 asks using RNAi MAX (Invitrogen, USA) as per the manufacturer's protocol. 62.5 nM of Syn-hsa-miR-92b-5p miScript miRNA mimic (Qiagen, Germany) was used for mimic transfection. All-Stars Negative Control siRNA (Qiagen, Germany) was used as mimic control. miRNA inhibition was done using 62.5 nM of miRCURY LNA miRNA inhibitor against has-miR-92b-5p (Qiagen, Germany) and miRCURY LNA miRNA Inhibitor Control (Qiagen, Germany) was used as the inhibitor control. Transfections were done using Opti-MEM media (Invitrogen, USA) for 5 h and replaced with complete MEM. Cells were processed after 48 hours of transfection for RNA and Protein analysis. Effective doses of mimic and inhibitor miRNA were standardized via qPCR and protein studies.
3
Transfection of Astrocytes with miR-92b-5p
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Transfection was done on astrocytes, in 80% con uent T25 asks using RNAi MAX (Invitrogen, USA) as per the manufacturer's protocol. 62.5 nM of Syn-hsa-miR-92b-5p miScript miRNA mimic (Qiagen, Germany) was used for mimic transfection. All-Stars Negative Control siRNA (Qiagen, Germany) was used as mimic control. miRNA inhibition was done using 62.5 nM of miRCURY LNA miRNA inhibitor against has-miR-92b-5p (Qiagen, Germany) and miRCURY LNA miRNA Inhibitor Control (Qiagen, Germany) was used as the inhibitor control. Transfections were done using Opti-MEM media (Invitrogen, USA) for 5 h and replaced with complete MEM. Cells were processed after 48 hours of transfection for RNA and Protein analysis. Effective doses of mimic and inhibitor miRNA were standardized via qPCR and protein studies.
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