Log In Sign up
The largest database of trusted experimental protocols

Orcaer hal100

Manufactured by Hamamatsu Photonics
Visit Supplier

The ORCA-ER HAL100 is a high-speed, low-noise, back-illuminated CMOS image sensor from Hamamatsu Photonics. It offers high quantum efficiency and fast readout speeds, making it suitable for a variety of scientific and industrial applications.

Automatically generated - may contain errors

Lab products found in correlation

Axiovert 200m, by Zeiss (2 mentions) Prolong gold, by Thermo Fisher Scientific (2 mentions) Formalin, by Avantor (2 mentions) Mcd19 2e2b6b10 antibody, by Abcam (2 mentions) Iview dab detection kit, by Abcam (2 mentions) Apex superior adhesive slides, by Leica (2 mentions)

2 protocols using orcaer hal100

1

Immunohistochemical Analysis of Murine Spleen and Liver

Check if the same lab product or an alternative is used in the 5 most similar protocols
Spleen and liver were obtained from experimental mice were immersed in 20 ml of formalin (VWR) for 24h and transferred to PBS. Paraffin embedding was carried out using standard procedures on a Tissue-TEK VIP processor (Miles Scientific), and 4-μm sections were mounted on Apex superior adhesive slides (Leica Microsystems) and stained on a Ventana BenchMark automated IHC stainer). mCD19 (2E2B6B10) antibody from Abcam was used and the antigen-antibody reaction was detected and visualized using the Ventana iView DAB detection kit, including secondary antibody and other necessary reagents. Immunohistochemistry sections were mounted with mounting medium, antifade reagent (Pro-Long Gold; Invitrogen) was applied, and coverslips were sealed before acquisition of fluorescent images at 25 degrees C on a Zeiss Axiovert 200M inverted confocal microscope with a 40 Plan Neofluor objective using IP Lab 4.0 software (Scanalytics). Photomicrographs were acquired using a Hamamatsu ORCAER HAL100 digital camera (400× original amplification).
Swaminathan S., Klemm L., Park E., Papaemmanuil E., Ford A., Kweon S.M., Trageser D., Hasselfeld B., Henke N., Mooster J., Geng H., Schwarz K., Kogan S.C., Casellas R., Schatz D.G., Lieber M.R., Greaves M.F, & Müschen M. (2015). Mechanisms of clonal evolution in childhood acute lymphoblastic leukemia. Nature immunology, 16(7), 766-774.
+ Open protocol
+ Expand
2

Immunohistochemical Analysis of Murine Spleen and Liver

Check if the same lab product or an alternative is used in the 5 most similar protocols
Spleen and liver were obtained from experimental mice were immersed in 20 ml of formalin (VWR) for 24h and transferred to PBS. Paraffin embedding was carried out using standard procedures on a Tissue-TEK VIP processor (Miles Scientific), and 4-μm sections were mounted on Apex superior adhesive slides (Leica Microsystems) and stained on a Ventana BenchMark automated IHC stainer). mCD19 (2E2B6B10) antibody from Abcam was used and the antigen-antibody reaction was detected and visualized using the Ventana iView DAB detection kit, including secondary antibody and other necessary reagents. Immunohistochemistry sections were mounted with mounting medium, antifade reagent (Pro-Long Gold; Invitrogen) was applied, and coverslips were sealed before acquisition of fluorescent images at 25 degrees C on a Zeiss Axiovert 200M inverted confocal microscope with a 40 Plan Neofluor objective using IP Lab 4.0 software (Scanalytics). Photomicrographs were acquired using a Hamamatsu ORCAER HAL100 digital camera (400× original amplification).
Swaminathan S., Klemm L., Park E., Papaemmanuil E., Ford A., Kweon S.M., Trageser D., Hasselfeld B., Henke N., Mooster J., Geng H., Schwarz K., Kogan S.C., Casellas R., Schatz D.G., Lieber M.R., Greaves M.F, & Müschen M. (2015). Mechanisms of clonal evolution in childhood acute lymphoblastic leukemia. Nature immunology, 16(7), 766-774.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!