Protoporphyrin 9 disodium salt ppix

In this experiment, the following photosensitizers were used: Rose Bengal (RB) disodium salt (95%) (Sigma Aldrich, St. Louis, MO, USA) at a concentration of 0.1 mM (5 samples were charged with 0.1 mM RB without illumination, 6 samples of 0.1 mM RB with illumination), 0 mM (5 samples, illuminated), 0.2 mM (6 samples, illuminated), 0.3 mM (6 samples, illuminated), 0.4 mM (6 samples, illuminated), and 0.5 mM (6 samples, illuminated); Protoporphyrin IX disodium salt (PpIX) (Sigma Aldrich, St. Louis, MO, USA) was used for 15 samples at a concentration of 3.0 mM PpIX with illumination, 5 samples with 3.0 mM PpIX without illumination, and 5 samples with 0 mM PpIX with illumination. The structures of RB and PpIX are depicted in Figure 6. Water for the preparation of the photosensitizer stock solutions was purified with a reverse osmosis water treatment system (AquaB Duo, Fresenius Medical Care, Singapore). The stock solutions of RB and PpIX were purged with oxygen (99%, STP & DIN Chemicals, Bielsko-Biała, Poland) for 10 min prior to their addition to the prostate tissue samples. The number of samples and the concentrations of photosensitizers used in addition to the prostate tissue samples that were either irradiated or kept in the dark are presented in Table 1.