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Rabbit a notch1

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Rabbit a-NOTCH1 is a primary antibody that recognizes the NOTCH1 protein. NOTCH1 is a transmembrane receptor that plays a crucial role in cell-cell signaling and cellular development. This antibody can be used to detect and quantify NOTCH1 expression in various experimental applications.

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Lab products found in correlation

Protease inhibitor, by Santa Cruz Biotechnology (2 mentions) Sensitive supersignal west femto maximum sensitivity substrate, by Thermo Fisher Scientific (2 mentions) Hrp anti mouse, by GE Healthcare (2 mentions) Western lightning plus ecl, by PerkinElmer (2 mentions)

Close spelling variants of this product

Notch1

2 protocols using rabbit a notch1

1

Analyzing Notch Signaling in Rhabdomyosarcoma

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Total cell lysates from human rhabdomyosarcoma cell lines and human myoblasts were obtained following lysis in 2% SDS lysis buffer supplemented with protease inhibitors (Santa Cruz Biotechnology). Western blot analysis used primary antibodies: rabbit a-NOTCH1 (Abcam), a-Cleaved NOTCH1 (Cell Signaling Technology), a-SNAI1 goat pAB (R&D Systems), a-Myosin Heavy Chain mouse mAb (monoclonal antibody) myosin heavy chain (a-MF20, R&D), MEF2C rabbit mAb (CST); and secondary antibodies: HRP (horseradish peroxidase) anti-rabbit (CST, 7074) or HRP anti-mouse (GE Healthcare, NA93IV). Membranes were developed using an ECL reagent (Western Lightning Plus ECL, PerkinElmer; or sensitive SuperSignal West Femto Maximum Sensitivity Substrate, Thermo Scientific). Membranes were striped, rinsed, and re-probed with the respective internal control rabbit a-Lamin B1 (Abcam) or rabbit a-GAPDH (CST).
Ignatius M.S., Hayes M.N., Lobbardi R., Chen E.Y., McCarthy K.M., Sreenivas P., Motala Z., Durbin A.D., Molodtsov A., Reeder S., Jin A., Sindiri S., Beleyea B.C., Bhere D., Alexander M.S., Shah K., Keller C., Linardic C.M., Nielsen P.G., Malkin D., Khan J, & Langenau D.M. (2017). The NOTCH1/SNAIL1/MEF2C Pathway Regulates Growth and Self-Renewal in Embryonal Rhabdomyosarcoma. Cell reports, 19(11), 2304-2318.
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2

Analyzing Notch Signaling in Rhabdomyosarcoma

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total cell lysates from human rhabdomyosarcoma cell lines and human myoblasts were obtained following lysis in 2% SDS lysis buffer supplemented with protease inhibitors (Santa Cruz Biotechnology). Western blot analysis used primary antibodies: rabbit a-NOTCH1 (Abcam), a-Cleaved NOTCH1 (Cell Signaling Technology), a-SNAI1 goat pAB (R&D Systems), a-Myosin Heavy Chain mouse mAb (monoclonal antibody) myosin heavy chain (a-MF20, R&D), MEF2C rabbit mAb (CST); and secondary antibodies: HRP (horseradish peroxidase) anti-rabbit (CST, 7074) or HRP anti-mouse (GE Healthcare, NA93IV). Membranes were developed using an ECL reagent (Western Lightning Plus ECL, PerkinElmer; or sensitive SuperSignal West Femto Maximum Sensitivity Substrate, Thermo Scientific). Membranes were striped, rinsed, and re-probed with the respective internal control rabbit a-Lamin B1 (Abcam) or rabbit a-GAPDH (CST).
Ignatius M.S., Hayes M.N., Lobbardi R., Chen E.Y., McCarthy K.M., Sreenivas P., Motala Z., Durbin A.D., Molodtsov A., Reeder S., Jin A., Sindiri S., Beleyea B.C., Bhere D., Alexander M.S., Shah K., Keller C., Linardic C.M., Nielsen P.G., Malkin D., Khan J, & Langenau D.M. (2017). The NOTCH1/SNAIL1/MEF2C Pathway Regulates Growth and Self-Renewal in Embryonal Rhabdomyosarcoma. Cell reports, 19(11), 2304-2318.
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